Distribution of 17β-hydroxysteroid dehydrogenases in human osteoblast-like cells

被引：21

作者：

Feix, M

Wolf, L

Schweikert, HU

机构：

[1] Univ Bonn, Dept Internal Med, D-53111 Bonn, Germany

[2] St Petrus Krankenhaus, Dept Orthoped, Bonn, Germany

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 2001年 / 171卷 / 1-2期

关键词：

17-beta-hydroxysteroid dehydrogenase isozymes; human osteoblast-like cells; bone;

D O I：

10.1016/S0303-7207(00)00393-2

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

In bone androgens and estrogens exert profound osteoprotective effects. Cultured human osteoblast (hOB)-like cells are able to metabolize circulating androgens or androgen precursors, such as testosterone and androstenedione, respectively, by aromatization (aromatase). 5 alpha -reduction (5 alpha -reductase) and reduction/oxidation at the 17 beta -position (17-beta -hydroxysteroid dehydrogenases, 17 beta -HSDs). In this study it was demonstrated that cultured normal human osteoblast-like cells as well as the osteosarcoma cell lines HOS and MG 63 express 17 beta -HSDs types 1. 2, 3 and 4. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

引用

收藏

页码：163 / 164

页数：2

相关论文

共 8 条

[1] ANDROSTENEDIONE METABOLISM IN CULTURED HUMAN OSTEOBLAST-LIKE CELLS [J].

BRUCH, HR ;

WOLF, L ;

BUDDE, R ;

ROMALO, G ;

SCHWEIKERT, HU .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1992, 75 (01) :101-105

[2] Human brain short chain L-3-hydroxyacyl coenzyme A dehydrogenase is a single-domain multifunctional enzyme -: Characterization of a novel 17β-hydroxysteroid dehydrogenase [J].

He, XY ;

Merz, G ;

Mehta, P ;

Schulz, H ;

Yang, SY .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (21) :15014-15019

[3] A human brain L-3-hydroxyacyl coenzyme A dehydrogenase is identical to an amyloid p-peptide-binding protein involved in Alzheimer's disease [J].

He, XY ;

Schulz, H ;

Yang, SY .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (17) :10741-10746

[4] Determination of cDNA, gene structure and chromosomal localization of the novel human 17β-hydroxysteroid dehydrogenase type 7 [J].

Krazeisen, A ;

Breitling, R ;

Imai, K ;

Fritz, S ;

Möller, G ;

Adamski, J .

FEBS LETTERS, 1999, 460 (02) :373-379

[5] 17β-Hydroxysteroid dehydrogenase (HSD)/17-ketosteroid reductase (KSR) family;: nomenclature and main characteristics of the 17HSD/KSR enzymes [J].

Peltoketo, H ;

Luu-The, V ;

Simard, J ;

Adamski, J .

JOURNAL OF MOLECULAR ENDOCRINOLOGY, 1999, 23 (01) :1-11

[6] Molecular endocrinology of hydroxysteroid dehydrogenases [J].

Penning, TM .

ENDOCRINE REVIEWS, 1997, 18 (03) :281-305

[7] TESTOSTERONE-METABOLISM IN HUMAN-BONE [J].

SCHWEIKERT, HU ;

RULF, W ;

NIEDERLE, N ;

SCHAFER, HE ;

KECK, E ;

KRUCK, F .

ACTA ENDOCRINOLOGICA, 1980, 95 (02) :258-264

[8] ESTROGEN FORMATION FROM ANDROSTENEDIONE IN HUMAN BONE [J].

SCHWEIKERT, HU ;

WOLF, L ;

ROMALO, G .

CLINICAL ENDOCRINOLOGY, 1995, 43 (01) :37-42