The regulatory effect of fermentable sugar levels on the production of leukotoxin by Actinobacillus actinomycetemcomitans

被引:23
作者
Mizoguchi, K
Ohta, H
Miyagi, A
Kurihara, H
Takashiba, S
Kato, K
Murayama, Y
Fukui, K
机构
[1] OKAYAMA UNIV,SCH DENT,DEPT MICROBIOL,OKAYAMA 700,JAPAN
[2] OKAYAMA UNIV,SCH DENT,DEPT PERIODONTOL & ENDODONTOL,OKAYAMA 700,JAPAN
关键词
Actinobacillus actinomycetemcomitans; leukotoxin; chemostat culture; sugar repression;
D O I
10.1016/S0378-1097(96)00473-9
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The relationship between sugar availability and RTX (repeats in toxin) cytotoxin (leukotoxin) production in the periodontopathic bacterium, Actinobacillus actinomycetemcomitans, was investigated using a chemostat. A. actinomycetemcomitans 301-b produced significant amounts of leukotoxin in anaerobic fructose-limited chemostat cultures at a dilution rate of 0.15 h(-1) and at pH 7.0. When the growth limitation was relieved by pulsing the cultures with 50 or 150 mM fructose (final concentrations), leukotoxin production immediately stopped and the amount of cellular leukotoxin decreased until the culture was returned to fructose-limited conditions. Leukotoxin synthesis was also repressed in the chemostat cultures by pulsing with glucose but not with the non-fermentable sugar analog, alpha-methyl-D-glucoside. Leukotoxin production was also repressed by fructose in chemostat cultures of ATCC 33384, which is generally recognized as a non-leukotoxin-producing or minimally leukotoxic strain.
引用
收藏
页码:161 / 166
页数:6
相关论文
共 16 条
[1]   REPRESSION OF HEAT-STABLE ENTEROTOXIN SYNTHESIS IN ENTEROTOXIGENIC ESCHERICHIA-COLI [J].
ALDERETE, JF ;
ROBERTSON, DC .
INFECTION AND IMMUNITY, 1977, 17 (03) :629-633
[2]   LEUKOTOXIC ACTIVITY IN DIFFERENT STRAINS OF THE BACTERIUM ACTINOBACILLUS-ACTINOMYCETEMCOMITANS ISOLATED FROM JUVENILE PERIODONTITIS IN MAN [J].
BAEHNI, PC ;
TSAI, CC ;
MCARTHUR, WP ;
HAMMOND, BF ;
SHENKER, BJ ;
TAICHMAN, NS .
ARCHIVES OF ORAL BIOLOGY, 1981, 26 (08) :671-676
[3]   REGULATION OF ACTINOBACILLUS-ACTINOMYCETEMCOMITANS LEUKOTOXIN EXPRESSION - ANALYSIS OF THE PROMOTER REGIONS OF LEUKOTOXIC AND MINIMALLY LEUKOTOXIC STRAINS [J].
BROGAN, JM ;
LALLY, ET ;
POULSEN, K ;
KILIAN, M ;
DEMUTH, DR .
INFECTION AND IMMUNITY, 1994, 62 (02) :501-508
[4]   PRODUCTION OF ESCHERICHIA-COLI STB ENTEROTOXIN IS SUBJECT TO CATABOLITE REPRESSION [J].
BUSQUE, P ;
LETELLIER, A ;
HAREL, J ;
DUBREUIL, JD .
MICROBIOLOGY-SGM, 1995, 141 :1621-1627
[5]  
HARA K, 1969, Journal of Periodontal Research, V4, P202, DOI 10.1111/j.1600-0765.1969.tb01968.x
[6]   NUCLEOTIDE-SEQUENCE OF THE LEUKOTOXIN GENE FROM ACTINOBACILLUS-ACTINOMYCETEMCOMITANS - HOMOLOGY TO THE ALPHA-HEMOLYSIN LEUKOTOXIN GENE FAMILY [J].
KRAIG, E ;
DAILEY, T ;
KOLODRUBETZ, D .
INFECTION AND IMMUNITY, 1990, 58 (04) :920-929
[7]   The relationships between leukotoxin production, growth rate and the bicarbonate concentration in a toxin-production-variable strain of Actinobacillus actinomycetemcomitans [J].
Ohta, H ;
Miyagi, A ;
Kato, K ;
Fukui, K .
MICROBIOLOGY-UK, 1996, 142 :963-970
[8]   LEUKOTOXIC ACTIVITY IN ACTINOBACILLUS (HAEMOPHILUS) ACTINOMYCETEMCOMITANS ISOLATED FROM PERIODONTAL-DISEASE PATIENTS [J].
OHTA, H ;
KOKEGUCHI, S ;
FUKUI, K ;
KATO, K .
MICROBIOLOGY AND IMMUNOLOGY, 1987, 31 (04) :313-325
[9]  
OHTA H, 1989, J GEN MICROBIOL, V135, P3485
[10]   ASSOCIATION OF ACTINOBACILLUS-ACTINOMYCETEMCOMITANS LEUKOTOXIN WITH NUCLEIC-ACIDS ON THE BACTERIAL-CELL SURFACE [J].
OHTA, H ;
HARA, H ;
FUKUI, K ;
KURIHARA, H ;
MURAYAMA, Y ;
KATO, K .
INFECTION AND IMMUNITY, 1993, 61 (11) :4878-4884