Potential sites of bioactive gibberellin production during reproductive growth in Arabidopsis

被引:185
作者
Hu, Jianhong [1 ]
Mitchum, Melissa G. [1 ]
Barnaby, Neel [1 ]
Ayele, Belay T. [2 ]
Ogawa, Mikihiro [3 ]
Nam, Edward [1 ]
Lai, Wei-Chu [1 ]
Hanada, Atsushi [2 ]
Alonso, Jose M. [4 ]
Ecker, Joseph R. [4 ]
Swain, Stephen M. [3 ]
Yamaguchi, Shinjiro [2 ]
Kamiya, Yuji [2 ]
Sun, Tai-Ping [1 ]
机构
[1] Duke Univ, Dept Biol, Durham, NC 27708 USA
[2] RIKEN, Plant Sci Ctr, Tsurumi Ku, Kanagawa 2300045, Japan
[3] CSIRO, Plant Ind, Merbein, Vic 3505, Australia
[4] Salk Inst Biol Studies, Plant Biol Lab, La Jolla, CA 92037 USA
关键词
D O I
10.1105/tpc.107.057752
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Gibberellin 3-oxidase (GA3ox) catalyzes the final step in the synthesis of bioactive gibberellins (GAs). We examined the expression patterns of all four GA3ox genes in Arabidopsis thaliana by promoter -beta-glucuronidase gene fusions and by quantitative RT-PCR and defined their physiological roles by characterizing single, double, and triple mutants. In developing flowers, GA3ox genes are only expressed in stamen filaments, anthers, and flower receptacles. Mutant plants that lack both GA3ox1 and GA3ox3 functions displayed stamen and petal defects, indicating that these two genes are important for GA production in the flower. Our data suggest that de novo synthesis of active GAs is necessary for stamen development in early flowers and that bioactive GAs made in the stamens and/or flower receptacles are transported to petals to promote their growth. In developing siliques, GA3ox1 is mainly expressed in the replums, funiculi, and the silique receptacles, whereas the other GA3ox genes are only expressed in developing seeds. Active GAs appear to be transported from the seed endosperm to the surrounding maternal tissues where they promote growth. The immediate upregulation of GA3ox1 and GA3ox4 after anthesis suggests that pollination and/or fertilization is a prerequisite for de novo GA biosynthesis in fruit, which in turn promotes initial elongation of the silique.
引用
收藏
页码:320 / 336
页数:17
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