Binding and two-dimensional crystallization of streptavidin at the air/water interface via engineered Cu-IDA chelator lipids

被引：9

作者：

Maloney, KM

Schief, WR

Pack, DW

Frey, W

Arnold, FH

Vogel, V

机构：

[1] Univ Washington, Dept Bioengn, Seattle, WA 98195 USA

[2] CALTECH, Dept Chem & Chem Engn, Pasadena, CA 91125 USA

来源：

COORDINATION CHEMISTRY REVIEWS | 1999年 / 183卷

基金：

美国国家航空航天局; 美国国家卫生研究院;

关键词：

air/water interface; Brewster angle microscopy; Cu(II); fluorescence microscopy; iminodiacetate; metal-chelating lipids; monolayers;

D O I：

10.1016/S0010-8545(98)00174-X

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

Metal-chelating iminodiacetate (IDA) lipid monolayers at the air/water interface have been engineered to promote the 2D crystallization of proteins which display solvent-accessible histidines. The physical properties of IDA lipid monolayers have been specifically tailored to allow the binding and 2D assembly of added proteins. Fluorescently labeled IDA lipid monolayers allow us to monitor the formation of the protein crystals optically. Here we review our efforts in the interfacial crystallization of streptavidin as monitored using fluorescence, Brewster angle and transmission electron microscopy and discuss future challenges in this area. (c) 1999 Elsevier Science S.A. All rights reserved.

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页码：3 / 18

页数：16

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