Detection of high-risk HPV types by the Hybrid Capture 2 test

被引：1

作者：

Terry, G

Ho, L

Londesborough, P

Cuzick, J

Mielzynska-Lohnas, I

Lorincz, A

机构：

[1] UCL, Dept Mol Pathol, London W1P 6DB, England

[2] Imperial Canc Res Fund, Dept Math Stat & Epidemiol, London WC2A 3PX, England

[3] Digene Corp, Gaithersburg, MD USA

来源：

JOURNAL OF MEDICAL VIROLOGY | 2001年 / 65卷 / 01期

关键词：

HC2-B; HPV; PCR;

D O I：

10.1002/jmv.2015.abs

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A hybrid capture test that can be used to detect at least 13 high-risk HPV types (referred to collectively as HPV-HR) in cervical scrapes (Hybrid Capture 2 probe set B, HC2-B) was evaluated. The HC2-B test is accurate and highly reproducible and the results obtained show excellent agreement with those obtained by a multiplexed type-specific polymerase chain reaction (mts-PCR). An additional assay to identify a subset of HPV-HR types may improve specificity without compromising sensitivity in HC2-B positive specimens with a test result close to the cut-off value given by 1 pg/ml HPV DNA. (C) 2001 Wiley-Liss, Inc.

引用

页码：155 / 162

页数：8

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