Enzymatic-spectrophotometric determination of sucrose in coffee beans

被引：21

作者：

Alcázar, A ^{[1
]}

Jurado, MJM ^{[1
]}

Martín, J ^{[1
]}

Pablos, F ^{[1
]}

González, AG ^{[1
]}

机构：

[1] Univ Seville, Fac Chem, Dept Analyt Chem, E-41012 Seville, Spain

来源：

TALANTA | 2005年 / 67卷 / 04期

关键词：

sucrose; coffee; enzymatic determination; spectrophotometry;

D O I：

10.1016/j.talanta.2005.04.005

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A spectrophotometric method for determining sucrose is proposed. Sucrose is hydrolyzed by invertase into glucose and fructose. Then, glucose is oxidized in presence of glucose oxidase and the produced hydrogen peroxide reacts with phenol-4-sulfonic acid sodium salt and 4-aminoantipyrine in presence of peroxidase, yielding a pink dye with an absorption maximum at 505 nm. This method was validated following the EURACHEM and VAM project guidelines for method validation. Trueness, precision, robustness, sensitivity and linearity were considered. The method was applied to the determination of sucrose in green and roasted coffee beans. A comparison with the HPLC method with pulsed amperometric detection was carried out. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：760 / 766

页数：7

共 27 条

[1]

[Anonymous], QUANT UNC AN MEAS

[2]

*ASTM, 1993, 898 ASTM E

[3]

*ASTM, 1991, 617 ASTM E

[4]

BABLOK W, 1988, J CLIN CHEM CLIN BIO, V26, P783

[5]

CLIFFORD M N, 1985, P305

[6] COMPOSITION OF GREEN COFFEE FRACTIONS AND THEIR CONTRIBUTION TO THE VOLATILE PROFILE FORMED DURING ROASTING [J].

DEMARIA, CAB ;

TRUGO, LC ;

MOREIRA, RFA ;

WERNECK, CC .

FOOD CHEMISTRY, 1994, 50 (02) :141-145

[7]

Ellison S.L.R, 2000, LGCVAM1998088

[8] Advances in methodology for the validation of methods according to the International Organization for Standardization Application to the determination of benzoic and sorbic acids in soft drinks by high-performance liquid chromatography [J].

García, I ;

Ortiz, MC ;

Sarabia, L ;

Vilches, C ;

Gredilla, E .

JOURNAL OF CHROMATOGRAPHY A, 2003, 992 (1-2) :11-27

[9] Two level factorial experimental designs based on multiple linear regression models:: A tutorial digest illustrated by case studies [J].

González, AG .

ANALYTICA CHIMICA ACTA, 1998, 360 (1-3) :227-241

[10]

HELRICH K, 1993, PEER VERIFIED METHOD

← 1 2 3 →