In vitro changes of the nuclear AgNORs pattern induced by RNA inhibitors and 5-fluorouracil in human breast cancer cells, MCF-7 and HBC-4

被引：2

作者：

Furuya, Y ^{[1
]}

Yamamoto, K ^{[1
]}

Kohno, N ^{[1
]}

Hamaoka, T ^{[1
]}

Ono, H ^{[1
]}

Kuroda, Y ^{[1
]}

机构：

[1] Kobe Univ, Sch Med, Dept Surg 1, Chuo Ku, Kobe, Hyogo 650, Japan

来源：

CANCER LETTERS | 1998年 / 134卷 / 01期

关键词：

breast cancer; rRNA; 5-fluorouracil; argyrophilic nucleolar organizer region (AgNOR); chemotherapy;

D O I：

10.1016/S0304-3835(98)00243-2

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

The morphological changes of argyrophilic nucleolar organizer regions (AgNORs) were studied in two human breast cancer cell lines, MCF-7 and HBC-4. Treatment with an RNA polymerase inhibitor (actinomycin D) reduced the size of AgNORs and increased the number of AgNORs. Messenger RNA polymerase inhibitor (alpha-amanitin) also increased the number of AgNORs. However, translational blocking agents closely related to ribosomal RNA (cycloheximide and anisomycin) caused a decrease in the number of AgNORs, which seemed to fuse to an aggregate around the nucleolus and formed a single large spherical AgNOR in the final stage. These changes were observed typically when cells were treated with 5-fluorouracil or 5-fluorouridine. These morphological changes in the AgNORs pattern, AgNORs aggregation, might reflect certain damage in ribosomal RNA. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：97 / 101

页数：5

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