UGT75L6 and UGT94E5 mediate sequential glucosylation of crocetin to crocin in Gardenia jasminoides

被引：73

作者：

Nagatoshi, Mai ^{[1
]}

Terasaka, Kazuyoshi ^{[1
]}

Owaki, Miki ^{[1
]}

Sota, Makiko ^{[1
]}

Inukai, Tatsunori ^{[1
]}

Nagatsu, Akito ^{[2
]}

Mizukami, Hajime ^{[1
]}

机构：

[1] Nagoya City Univ, Grad Sch Pharmaceut Sci, Nagoya, Aichi, Japan

[2] Kinjo Gakuin Univ, Sch Pharm, Yokohama, Kanagawa, Japan

来源：

FEBS LETTERS | 2012年 / 586卷 / 07期

关键词：

Apocarotenoid; Crocin; Glucosyltransferase; Gardenia jasminoides; UDP-GLUCOSE; GLUCOSYLTRANSFERASE; SATIVUS; GLYCOSYLTRANSFERASES; IDENTIFICATION; BIOSYNTHESIS; DIOXYGENASES; STIGMAS; EXTRACT; CLONING;

D O I：

10.1016/j.febslet.2012.03.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Crocin is an apocarotenoid glycosyl ester accumulating in fruits of Gardenia jasminoides and used as a food coloring and nutraceutical. For the first time, the two glucosyltransferases UGT75L6 and UGT94E5 that sequentially mediate the final glucosylation steps in crocin biosynthesis in G. jasminoides have been identified and functionally characterized. UGT75L6 preferentially glucosylates the carboxyl group of crocetin yielding crocetin glucosyl esters, while UGT94E5 glucosylates the 60 hydroxyl group of the glucose moiety of crocetin glucosyl esters. The expression pattern of neither UGT75L6 nor UGT94E5 correlated with the pattern of crocin accumulation in G. jasminoides. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：1055 / 1061

页数：7

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