Bone marrow stromal cells regulate caspase 3 activity in leukemic cells during chemotherapy

被引:48
作者
Fortney, JE
Zhao, W
Wenger, SL
Gibson, LF
机构
[1] W Virginia Univ, Dept Pediat, Hlth Sci Ctr, Morgantown, WV 26506 USA
[2] W Virginia Univ, Hlth Sci Ctr, Dept Pathol, Morgantown, WV 26506 USA
[3] W Virginia Univ, Hlth Sci Ctr, Dept Microbiol & Immunol, Morgantown, WV 26506 USA
[4] W Virginia Univ, Mary Babb Randolph Canc Ctr, Blood & Marrow Transplantat Program, Morgantown, WV 26506 USA
关键词
caspase; 3; acute lymphoblastic leukemia; stromal cells; apoptosis; chemotherapy;
D O I
10.1016/S0145-2126(01)00051-0
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The interaction between leukemic cells and stromal cells of the bone marrow microenvironment has been shown to enhance leukemic cell survival during exposure to chemotherapeutic agents. In the current study we investigated whether association of B lineage acute lymphoblastic leukemic cells with human bone marrow stromal cells altered caspase activation during chemotherapy treatment. Following treatment with Ara-C or VP-16 in vitro, caspase 3 activity in leukemic cells was consistently reduced by co-culture of leukemic cells with human bone marrow stromal cell layers. These observations suggest that the protective effect of the bone marrow microenvironment on leukemic cells may be due, in part, to regulation of caspase 3 activity. (C) 2001 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:901 / 907
页数:7
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