A-kinase anchoring protein 12 regulates the completion of cytokinesis

被引：13

作者：

Choi, Moon-Chang ^{[2
]}

Lee, Yang-Ui ^{[2
]}

Kim, Sung-Hak ^{[2
]}

Park, Jung-Hyun ^{[2
]}

Kim, Hyun-Ah ^{[2
]}

Oh, Do-Youn ^{[1
,2
]}

Im, Seock-Ah ^{[1
,2
]}

Kim, Tae-You ^{[1
,2
]}

Jong, Hyun-Soon ^{[2
]}

Bang, Yung-Jue ^{[1
,2
]}

机构：

[1] Seoul Natl Univ, Coll Med, Dept Internal Med, Seoul 110799, South Korea

[2] Canc Res Inst, Natl Res Lab Canc Epigenet, Seoul, South Korea

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 373卷 / 01期

基金：

新加坡国家研究基金会;

关键词：

AKAP; AKAP12; gravin; cytokinesis; MLCK; ML-9;

D O I：

10.1016/j.bbrc.2008.05.184

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A-kinase anchoring protein 12 (AKAP12) gene is frequently inactivated in human gastric cancer and in several other cancers due to promoter hypermethylation. However, the biological function of AKAP12 in tumorigenesis remains to be identified. Aneuploidy, a hallmark of cancer cells, is often caused by abnormal cell division. In the present study, AKAP12 was found to localize to the cell periphery during interphase and to the actomyosin contractile ring during cytokinesis. Furthermore, AKAP12 depletion using small interfering RNA increased the number of multinucleated cells, and disrupted the completion of cytokinesis. Interestingly, the inhibition of myosin light chain kinase (MLCK), a key regulator of actomyosin contractility, removed AKAP12 from the cell periphery during interphase and from the contractile ring during cytokinesis, suggesting that AKAP12 might be a downstream effector of MLCK. Our findings implicate AKAP12 in the regulation of cytokinesis progression, and suggest a novel role for AKAP12 tumor suppressor. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：85 / 89

页数：5

共 33 条

[1]

A-kinase anchoring proteins take shape [J].

Beene, Darren L. ;

Scott, John D. .

CURRENT OPINION IN CELL BIOLOGY, 2007, 19 (02) :192-198

[2]

Low expression of the putative tumour suppressor gene gravin in chronic myeloid leukaemia, myelodysplastic syndromes and acute myeloid leukaemia [J].

Boultwood, J ;

Pellagatti, A ;

Watkins, F ;

Campbell, LJ ;

Esoof, N ;

Cross, NCP ;

Eagleton, H ;

Littlewood, TJ ;

Fidler, C ;

Wainscoat, JS .

BRITISH JOURNAL OF HAEMATOLOGY, 2004, 126 (04) :508-511

[3]

The cellular geography of aurora kinases [J].

Carmena, M ;

Earnshaw, WC .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2003, 4 (11) :842-854

[4]

AKAP12/Gravin is inactivated by epigenetic mechanism in human gastric carcinoma and shows growth suppressor activity [J].

Choi, MC ;

Jong, HS ;

Kim, TY ;

Song, SH ;

Lee, DS ;

Lee, JW ;

Kim, TY ;

Kim, NK ;

Bang, YJ .

ONCOGENE, 2004, 23 (42) :7095-7103

[5]

Expression of a down-regulated target, SSeCKS, reverses v-Jun-induced transformation of 10T1/2 murine fibroblasts [J].

Cohen, SB ;

Waha, A ;

Gelman, IH ;

Vogt, PK .

ONCOGENE, 2001, 20 (02) :141-146

[6]

Distinct pathways control recruitment and maintenance of myosin II at the cleavage furrow during cytokinesis [J].

Dean, SO ;

Rogers, SL ;

Stuurman, N ;

Vale, RD ;

Spudich, JA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (38) :13473-13478

[7]

Diviani D, 2001, J CELL SCI, V114, P1431

[8]

Animal cytokinesis: From parts list to mechanisms [J].

Eggert, Ulrike S. ;

Mitchison, Timothy J. ;

Field, Christine M. .

ANNUAL REVIEW OF BIOCHEMISTRY, 2006, 75 :543-566

[9]

Parallel chemical genetic and genome-wide RNAi screens identify cytokinesis inhibitors and targets [J].

Eggert, US ;

Kiger, AA ;

Richter, C ;

Perlman, ZE ;

Perrimon, N ;

Mitchison, TJ ;

Field, CM .

PLOS BIOLOGY, 2004, 2 (12) :2135-2143

[10]

The biological functions of A-kinase anchor proteins [J].

Feliciello, A ;

Gottesman, ME ;

Avvedimento, EV .

JOURNAL OF MOLECULAR BIOLOGY, 2001, 308 (02) :99-114

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