Identification of two forms of the γ subunit of G protein, γ10 and γ11, in bovine lung and their tissue distribution in the rat

被引：25

作者：

Morishita, R ^{[1
]}

Ueda, H ^{[1
]}

Kato, K ^{[1
]}

Asano, T ^{[1
]}

机构：

[1] Aichi Human Serv Ctr, Inst Dev Res, Dept Biochem, Aichi 4800392, Japan

来源：

FEBS LETTERS | 1998年 / 428卷 / 1-2期

关键词：

G protein gamma subunit; purification; antibody; localization;

D O I：

10.1016/S0014-5793(98)00498-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two forms of the gamma subunit of G protein were purified from bovine lung, and were identified as gamma(10) and gamma(11) by analyses of partial amino acid sequences and reactivity with specific antibodies. The N-terminal amino acid residue of gamma(11) was an unmodified Pro(2), and the purified gamma(11) was freed from beta even under non-denaturing conditions, Western blots with specific antibodies against gamma(10) and gamma(11) showed that both gamma subunits are present in a variety of tissues in the rat, with a particular abundance of gamma(11) in the platelets. (C) 1998 Federation of European Biochemical Societies.

引用

页码：85 / 88

页数：4

共 25 条

[1] ASANO T, 1995, J NEUROCHEM, V64, P1267
[2] ASANO T, 1993, J BIOL CHEM, V268, P20512
[3] CALI JJ, 1992, J BIOL CHEM, V267, P24023
[4] FARNESYLATED GAMMA-SUBUNIT OF PHOTORECEPTOR G-PROTEIN INDISPENSABLE FOR GTP-BINDING
FUKADA, Y
TAKAO, T
OHGURO, H
YOSHIZAWA, T
AKINO, T
SHIMONISHI, Y
[J]. NATURE, 1990, 346 (6285) : 658 - 660
[5] G PROTEIN DIVERSITY IS INCREASED BY ASSOCIATIONS WITH A VARIETY OF GAMMA-SUBUNITS
GAUTAM, N
NORTHUP, J
TAMIR, H
SIMON, MI
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (20) : 7973 - 7977
[6] ISOLATION AND CHARACTERIZATION OF A CDNA CLONE FOR THE GAMMA-SUBUNIT OF BOVINE RETINAL TRANSDUCIN
HURLEY, JB
FONG, HKW
TEPLOW, DB
DREYER, WJ
SIMON, MI
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (22): : 6948 - 6952
[7] INIGUEZLLUHI JA, 1992, J BIOL CHEM, V267, P23409
[8] A BRAIN-SPECIFIC G-PROTEIN GAMMA-SUBUNIT
KALYANARAMAN, S
KALYANARAMAN, V
GAUTAM, N
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1995, 216 (01) : 126 - 132
[9] IDENTIFICATION AND ISOLATION OF COMMON AND TISSUE-SPECIFIC GERANYLGERANYLATED GAMMA-SUBUNITS OF GUANINE-NUCLEOTIDE-BINDING REGULATORY PROTEINS IN VARIOUS TISSUES
MORISHITA, R
FUKUDA, Y
KOKAME, K
YOSHIZAWA, T
MASUDA, K
NIWA, M
KATO, K
ASANO, T
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1992, 210 (03): : 1061 - 1069
[10] IDENTIFICATION OF 3 FORMS OF THE GAMMA-SUBUNIT OF G-PROTEINS ISOLATED FROM BOVINE SPLEEN
MORISHITA, R
MASUDA, K
NIWA, M
KATO, K
ASANO, T
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1993, 194 (03) : 1221 - 1227

← 1 2 3 →