Lymphocytes subsets in normal individuals: Analysis by four color immunofluorescence and flow cytometry on whole blood

被引：24

作者：

Schiavon, V

Roth, P

Bolton, WE

Farcet, JP

Bensussan, A

Boumsell, L

机构：

[1] FAC MED CRETEIL,INSERM U448,LAB IMMUNOL BIOL,F-94010 CRETEIL,FRANCE

[2] COULTER TECHNOL CTR,MIAMI,FL

来源：

TISSUE ANTIGENS | 1996年 / 48卷 / 4-I期

关键词：

CD; cytometry; four-color immunofluorescence; lymphocytes; subsets;

D O I：

10.1111/j.1399-0039.1996.tb02650.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

In order to precisely define human lymphocytes subsets, we used four color immunofluorescence analysis and flaw cytometry. We report here the results of systematic studies performed on whole blood from 20 normal volunteers. This was performed using monoclonal antibodies recognizing 18 different CD molecules and analyzed here into 14 different 4-color immunofluorescence combinations. This showed that among T cells, the two major CD3+CD8+ and CD3+CD8- subsets differed greatly in the percentage of cells expressing CD26, CD27, CD28, CD38, CD45RA, CD45RO, CD57, S6F1, BY55, CD101 and their respective combination representing an array of subsets. Furthermore the minor NK subsets, i.e. CD2+CD3-CD8+, CD2-CD3-CD8+, CD2+CD3-CD8-, CD2-CD3-CD8- also differed in percentages of cells expressing CD16, CD56, CD57, S6F1, BY55 and their combinations.

引用

页码：312 / 318

页数：7

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