Isolation and characterization of diazotrophic growth promoting bacteria from rhizosphere of agricultural crops of Korea

被引：180

作者：

Park, M

Kim, C

Yang, J

Lee, H

Shin, W

Kim, S

Sa, T ^{[1
]}

机构：

[1] Chungbuk Natl Univ, Dept Agr Chem, Cheongju 361763, Chungbuk, South Korea

[2] Natl Inst Agr Sci & Technol, Div Organ Farming Technol, Suwon 441747, South Korea

来源：

MICROBIOLOGICAL RESEARCH | 2005年 / 160卷 / 02期

关键词：

diazotrophs; acetytene reduction assay; 16S rDNA sequence; indole-3-acetic acid;

D O I：

10.1016/j.micres.2004.10.003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

plant namely sesame, maize, wheat, soybean, lettuce, pepper and rice grown in Chungbuk Province, Korea. Five isolates with nitrogenase activity above 150 nmol h(-1) mg(-1) protein were identified based on phenotypic and 16S rDNA sequences analysis. The strains were identified as Stenotrophomonas maltophilia (PM-1, PM-26), Bacillus fusiformis (PM-5, PM-24) and Pseudomonas fluorescens (PM-13), respectively. All the isotates produced indole-3-acetic acid (IAA), in the presence of tryptophan, ranging from 100.4 mu g ml(-1) (PM-13) to 255 mu g Ml(-1) (PM-24). The isolate PM-24 (Bacillus fusiformis) exhibiting highest nitrogenase activity (3677.81 nmol h(-1) mg(-1) protein) and IAA production (255 mu g ml(-1)) has a promising potential for developing as a plant growth promoting rhizobacteria. (c) 2004 Elsevier GrnbH. All rights reserved.

引用

页码：127 / 133

页数：7

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