Alginate lyase (AlgL) activity is required for alginate biosynthesis in Pseudomonas aeruginosa

被引：46

作者：

Albrecht, MT ^{[1
]}

Schiller, NL ^{[1
]}

机构：

[1] Univ Calif Riverside, Div Biomed Sci, Riverside, CA 92521 USA

来源：

JOURNAL OF BACTERIOLOGY | 2005年 / 187卷 / 11期

关键词：

D O I：

10.1128/JB.187.11.3869-3872.2005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

To determine whether AlgL's lyase activity is required for alginate production in Pseudomonas aeruginosa, an algL Delta::Gm(r) mutant (FRD-MA7) was created. algL complementation of FRD-MA7 restored alginate production, but algL constructs containing mutations inactivating lyase activity did not, demonstrating that the enzymatic activity of AlgL is required for alginate production.

引用

页码：3869 / 3872

页数：4

共 27 条

[1] GENETIC-ANALYSIS OF THE ALGINATE BIOSYNTHETIC GENE-CLUSTER OF PSEUDOMONAS-AERUGINOSA SHOWS EVIDENCE OF AN OPERONIC STRUCTURE [J].

CHITNIS, CE ;

OHMAN, DE .

MOLECULAR MICROBIOLOGY, 1993, 8 (03) :583-590

[2] GENDER DIFFERENCES IN CYSTIC-FIBROSIS - PSEUDOMONAS-AERUGINOSA INFECTION [J].

DEMKO, CA ;

BYARD, PJ ;

DAVIS, PB .

JOURNAL OF CLINICAL EPIDEMIOLOGY, 1995, 48 (08) :1041-1049

[3] REPLICATION OF AN ORIGIN-CONTAINING DERIVATIVE OF PLASMID RK2 DEPENDENT ON A PLASMID FUNCTION PROVIDED IN TRANS [J].

FIGURSKI, DH ;

HELINSKI, DR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (04) :1648-1652

[4] IDENTIFICATION OF ALGF IN THE ALGINATE BIOSYNTHETIC GENE-CLUSTER OF PSEUDOMONAS-AERUGINOSA WHICH IS REQUIRED FOR ALGINATE ACETYLATION [J].

FRANKLIN, MJ ;

OHMAN, DE .

JOURNAL OF BACTERIOLOGY, 1993, 175 (16) :5057-5065

[5] PSEUDOMONAS-AERUGINOSA ALGG IS A POLYMER LEVEL ALGINATE C5-MANNURONAN EPIMERASE [J].

FRANKLIN, MJ ;

CHITNIS, CE ;

GACESA, P ;

SONESSON, A ;

WHITE, DC ;

OHMAN, DE .

JOURNAL OF BACTERIOLOGY, 1994, 176 (07) :1821-1830

[6] Mutant analysis and cellular localization of the AlgI, AlgJ, and AlgF proteins required for O acetylation of alginate in Pseudomonas aeruginosa [J].

Franklin, MJ ;

Ohman, DE .

JOURNAL OF BACTERIOLOGY, 2002, 184 (11) :3000-3007

[7] Identification of algI and algJ in the Pseudomonas aeruginosa alginate biosynthetic gene cluster which are required for alginate O acetylation [J].

Franklin, MJ ;

Ohman, DE .

JOURNAL OF BACTERIOLOGY, 1996, 178 (08) :2186-2195

[8] The Pseudomonas fluorescens AlgG protein, but not its mannuronan C-5-epimerase activity, is needed for alginate polymer formation [J].

Gimmestad, M ;

Sletta, H ;

Ertesvåg, H ;

Bakkevig, K ;

Jain, S ;

Suh, S ;

Skjåk-Bræk, G ;

Ellingsen, TE ;

Ohman, DE ;

Valla, S .

JOURNAL OF BACTERIOLOGY, 2003, 185 (12) :3515-3523

[9] SYNTHESIS OF LIPOPOLYSACCHARIDE-O SIDE-CHAINS BY PSEUDOMONAS-AERUGINOSA PAO1 REQUIRES THE ENZYME PHOSPHOMANNOMUTASE [J].

GOLDBERG, JB ;

HATANO, K ;

PIER, GB .

JOURNAL OF BACTERIOLOGY, 1993, 175 (06) :1605-1611

[10] Pathogenicity of microbes associated with cystic fibrosis [J].

Hutchison, ML ;

Govan, JRW .

MICROBES AND INFECTION, 1999, 1 (12) :1005-1014

← 1 2 3 →