Semi-automated synthesis and screening of a large library of degradable cationic polymers for gene delivery

被引：374

作者：

Anderson, DG

Lynn, DM

Langer, R

机构：

[1] MIT, Dept Chem Engn, Cambridge, MA 02139 USA

[2] Univ Wisconsin, Dept Biol & Chem Engn, Madison, WI 53706 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2003年 / 42卷 / 27期

关键词：

DNA; gene therapy; high-throughput screening; polycations; polymers;

D O I：

10.1002/anie.200351244

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Higher delivery efficiency than conventional nonviral systems (such as poly(ethyleneimine)) have been identified in 46 polymers through cell-based screening of a large, 2350-member library by a high-throughput, semi-automated process. The transfection potential of the polymers was assessed by testing their ability to deliver luciferase expressing plasmid and green fluorescent protein plasmid (see picture) to a monkey kidney fibroblast cell line.

引用

页码：3153 / 3158

页数：6

共 18 条

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