Loop-mediated isothermal amplification for rapid detection of Bacillus anthracis spores

被引：64

作者：

Yan-Mei Qiao

Yong-Chao Guo

Xian-En Zhang ^{[1
]}

Ya-Feng Zhou

Zhi-Ping Zhang

Hong-Ping Wei

Rui-Fu Yang

Dian-Bing Wang

机构：

[1] Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China

[2] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China

[3] Acad Mil Med Sci, Inst Microbiol & Epidemiol, Beijing 100071, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2007年 / 29卷 / 12期

关键词：

bacillus anthracis; detection; isothermal amplification; LAMP;

D O I：

10.1007/s10529-007-9472-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A loop-mediated isothermal amplification (LAMP) assay system was employed for detecting Bacillus anthracis spores in pure cultures as well as in various simulated powder samples. The specificity of the designed LAMP primer sets was validated by assaying 13 B. anthracis strains and 33 non-B. anthracis species. The detection limits of the LAMP assay were 10 spores/tube for pure cultures and 100 spores/2 mg powder for simulated powder samples. The results show that the LAMP protocol is a promising method for detecting B. anthracis.

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收藏

页码：1939 / 1946

页数：8

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