Karyotyping of human synaptonemal complexes by cenM-FISH

被引：31

作者：

Oliver-Bonet, M ^{[1
]}

Liehr, T

Nietzel, A

Heller, A

Starke, H

Claussen, U

Codina-Pascual, M

Pujol, A

Abad, C

Egozcue, J

Navarro, J

Benet, J

机构：

[1] Univ Autonoma Barcelona, Fac Med, Dept Biol Cellular Fisiol & Immunol, Unitat Biol, Bellaterra 08193, Spain

[2] Inst Human Genet & Anthropol, D-07740 Jena, Germany

[3] Consorci Hosp Parc Tauli, Sabadell 08208, Spain

来源：

EUROPEAN JOURNAL OF HUMAN GENETICS | 2003年 / 11卷 / 11期

关键词：

cenM-FISH on synaptonemal complexes; immunocytogenetics; meiotic recombination;

D O I：

10.1038/sj.ejhg.5201067

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The purpose of this work was to adapt the recently described centromere-specific multicolour (cenM-) FISH technique to human meiotic cells, and evaluate the usefulness of this multiplex fluorescence method for karyotyping human synaptonemal complex (SC), previously analysed by immunocytogenetic approaches. The results obtained demonstrate that cenM- FISH is a reliable one-single-step method, which allows for the identification of all SC present in pachytene spreads. Moreover, when cenM- FISH is applied after immunocytogenetic analysis, the number and distribution of MLH1 foci per chromosome can be established and recombination analysis for each chromosome can be performed easily.

引用

页码：879 / 883

页数：5

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