Determination of glutamate by high-performance liquid chromatography with fluorescence detection in superfusates of rat brain preparations

被引：7

作者：

Ioannou, N

Georgopoulos, A

Liapi, C

Palaiologos, G

机构：

[1] Univ Athens, Sch Med, Biol Chem Lab, GR-11527 Athens, Greece

[2] Univ Athens, Sch Med, Pharmacol Lab, GR-11527 Athens, Greece

来源：

ANALYTICAL BIOCHEMISTRY | 1998年 / 264卷 / 01期

关键词：

D O I：

10.1006/abio.1998.2792

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is described for the determination of glutamate in superfusates of cortical or hippocampal rat brain slices. This method is based on the precolumn derivatization of amino acids with orthopthaldialdehyde and mercaptopropionic acid, separation by highperformance liquid chromatography, and detection by fluorescence. By adjusting the concentrations of reagents and with the minimum dilution of the sample, it was possible to reproducibly measure the Ca2+-dependent K+-evoked release of neurotransmitter glutamate in superfusates of brain slices. (C) 1998 Academic Press.

引用

页码：82 / 86

页数：5

共 5 条

[1] State-of-the-art of high-performance liquid chromatographic analysis of amino acids in physiological samples [J].