Tailor-made dyes for fluorescence correlation spectroscopy (FCS)

被引：12

作者：

Czerney, P

Lehmann, F

Wenzel, M

Buschmann, V

Dietrich, A

Mohr, GJ

机构：

[1] Dyom GmbH, Bioinstrumentezentrum, D-07745 Jena, Germany

[2] Univ Heidelberg, Inst Chem Phys, D-69120 Heidelberg, Germany

[3] ETH Technopk, Ctr Chem Sensors, CH-8005 Zurich, Switzerland

来源：

BIOLOGICAL CHEMISTRY | 2001年 / 382卷 / 03期

关键词：

diode lasers; fluorescent labels; life time; quantum yield;

D O I：

10.1515/BC.2001.062

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two new fluorescent labels are presented that are optimized for excitation with He/Ne laser and red diode lasers. Application in FCS and labeling of proteins and oligomers are demonstrated. A strong rise of quantum yield and emission life time upon binding to biomolecules are characteristic features of the dyes.

引用

页码：495 / 498

页数：4

共 9 条

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