Expression of H type 1 antigen of ABO histo-blood group in normal colon and aberrant expressions of H type 2 and H type 3/4 antigens in colon cancer

被引:23
作者
Fujitani, N
Liu, Y
Toda, S
Shirouzu, K
Okamura, T
Kimura, H
机构
[1] Kurume Univ, Sch Med, Dept Forens Med & Human Genet, Kurume, Fukuoka 8300011, Japan
[2] Okayama Univ Sci, Fac Sci, Dept Biochem, Okayama 7000005, Japan
[3] Saga Med Sch, Dept Pathol, Saga 8498501, Japan
[4] Kurume Univ, Sch Med, Dept Surg, Kurume, Fukuoka 8300011, Japan
关键词
colon; colon cancer; H antigen; secretor; ABO; anti-H antibody;
D O I
10.1023/A:1007173722426
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have immunohistochemically examined the distribution of the H antigens of type 1, type 2 and type 3/4 chains of the ABO(H) histo-blood group system in human normal colon and in colon cancer using three monoclonal antibodies specific for each of the H type 1/2, H type 2, and the H type 3/4 chain. We unexpectedly found that mucosa of the normal colon from secretors but not that from nonsecretors expressed only H type 1 and did not express H type 2 or H type 3/4. The H type 1 was expressed in goblet cells. Positive goblet cells expressing H type 1 were decreased in number progressively from the proximal colon to the rectum. In tumors, 4 (57%) of 7 cancer tissues of the proximal colon from secretors expressed no H type 1, whereas all 8 cancer tissues of the distal colon from secretors expressed H type 1. The aberrant expressions of H type 2 and H type 3/4 (47 and 67%, respectively) were found in cancer tissues from both the proximal and the distal colon. Tumors from nonsecretors did not express any H antigens. Our results suggested that the expression of H type 1 in the normal colon and the aberrant expressions of H type 2 and H type 3/4 in colon cancer tissues were regulated by FUT2-encoded Se type alpha (1,2)fucosyltransferase. However, UEA-I-positive substance(s) rather than H type 2 were uniquely expressed throughout the normal colon and in colon cancers from both secretors and nonsecretors.
引用
收藏
页码:331 / 338
页数:8
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