Functional characteristics of chlorophyll d-predominating photosynthetic apparatus in intact cells of Acaryochloris marina

被引:37
作者
Boichenko, VA [1 ]
Klimov, VV
Miyashita, H
Miyachi, S
机构
[1] Russian Acad Sci, Inst Basic Biol Problems, Pushchino 142290, Moscow Region, Russia
[2] Marine Biotechnol Inst, Kamaishi Labs, Kamaishi, Iwate 026, Japan
[3] Marine Biotechnol Inst, Bunkyo Ku, Tokyo 113, Japan
基金
俄罗斯基础研究基金会;
关键词
action spectra; antenna size; flash yield; H-2; photoevolution; O-2; evolution; photoinhibition of respiration; Photosystems I and II;
D O I
10.1023/A:1010637631417
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Functional organization of the photosynthetic apparatus in the unique chlorophyll d-predominating prokaryote, Acaryochloris marina, was studied using polarographic measurements of single-turnover flash yields, action spectra and optical cross sections for PS-specific reactions. O-2 evolution was indicative of PS II activity, while reversible photoinhibition of respiratory O-2 uptake under aerobic conditions in the presence of DCMU and H-2 photoevolution by anaerobically adapted cells were the indicatives of PS I activity. O-2 evolution in the cells upon single-turnover flashes followed the normal S-state cycle with a period-4 oscillation. Analysis of action spectra for the partial reactions of photosynthesis revealed that: (1) distinct spectral forms of Chl d are nonuniformly distributed between PS I and PS II, e.g. Chl d-695 and Chl d-735 are preferentially located in PS II and PS I, respectively; (2) a minor fraction of Chl a in the cells belongs mostly to PS II; (3) biliproteins transfer excitation energy both to PS II and, with a lower efficiency, PS I; (4) the efficiency of energy transfer from biliproteins to PS II depends on the light quality growth conditions and is larger in white light (WL)-grown cells compared to the red light (RL)-grown cells. Content of functional O-2 evolving PS II centers decreases 2 times in the RL-grown cells relative to the WL-grown cells, whereas content of competent PS I centers involved in photoinhibition of respiration remains almost the same in both the cultures. The effective antenna size of PS I was estimated to be 80-90 Chl d including 3-10 molecules absorbing at 735 nm. The effective optical cross-section of PS II corresponded to 90-100 Chl d and, presumably, 4 Chl a + 2 Pheo a [Mimuro et al. (1999) Biochim Biophys Acta 1412: 37-46]. Optical cross-section measurements indicated that the functional PS II units of A. marina attach one rod of four hexameric units of biliproteins.
引用
收藏
页码:269 / 277
页数:9
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