Effects of supplementing the feed to Atlantic cod (Gadus morhua) fry with lactic acid bacteria and immuno-stimulating peptides during a challenge trial with Vibrio anguillarum

被引:117
作者
Gildberg, A [1 ]
Mikkelsen, H [1 ]
机构
[1] Norwegian Inst Fisheries & Aquaculture, N-9005 Tromso, Norway
关键词
immune-stimulating peptides; probiotics; Carnobacterium divergens; Atlantic cod; intestinal colonisation;
D O I
10.1016/S0044-8486(98)00296-8
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
Atlantic cod (Gadus morhua) fry were reared on a commercial feed supplemented with Carnobacterium divergens, isolated from the intestines of Atlantic cod and Atlantic salmon (Salmo salar) alone or in combination with immuno-stimulating peptides. In vitro experiments showed that culture filtrates from the isolates from cod and salmon inhibited the growth of Vibrio anguillarum. After 3 weeks of feeding, the fish were challenged by bath exposure to V. anguillarum (10(7)/ml, 1 h). Twelve days after infection significant (p < 0.05) reduced cumulative mortality was recorded in fish given feed supplemented with lactic acid bacteria isolated from Atlantic salmon and with immune-stimulating peptides. No synergistic or cumulative effects were achieved by combining lactic acid bacteria and immune-stimulating peptides, Four weeks after infection the same cumulative mortality (80-84%) was reached in all groups. Both strains of lactic acid bacteria could colonize the internal mucus layer of the cod fry pyloric caeca, and a significant number of the bacteria survived the passage of the whole gastrointestinal tract. (C) 1998 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:103 / 113
页数:11
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