Osteoblast Production by Reserved Progenitor Cells in Zebrafish Bone Regeneration and Maintenance

被引：60

作者：

Ando, Kazunori ^{[1
]}

Shibata, Eri ^{[1
]}

Hans, Stefan ^{[2
]}

Brand, Michael ^{[2
]}

Kawakami, Atsushi ^{[1
]}

机构：

[1] Tokyo Inst Technol, Sch Life Sci & Technol, Midori Ku, 4259 Nagatsuta, Yokohama, Kanagawa 2268501, Japan

[2] DFG Ctr Regenerat Therapies Dresden, Dev Genet, Tatzberg 47-49, D-01307 Dresden, Germany

来源：

DEVELOPMENTAL CELL | 2017年 / 43卷 / 05期

关键词：

GENE-EXPRESSION; TISSUE; MEDAKA; DEDIFFERENTIATION; BLASTEMA;

D O I：

10.1016/j.devcel.2017.10.015

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

Mammals cannot re-form heavily damaged bones as in large fracture gaps, whereas zebrafish efficiently regenerate bones even after amputation of appendages. However, the source of osteoblasts that mediate appendage regeneration is controversial. Several studies in zebrafish have shown that osteoblasts are generated by dedifferentiation of existing osteoblasts at injured sites, but other observations suggest that de novo production of osteoblasts also occurs. In this study, we found from cell-lineage tracing and ablation experiments that a group of cells reserved in niches serves as osteoblast progenitor cells (OPCs) and has a significant role in fin ray regeneration. Besides regeneration, OPCs also supply osteoblasts for normal bone maintenance. We further showed that OPCs are derived from embryonic somites, as is the case with embryonic osteoblasts, and are replenished from mesenchymal precursors in adult zebrafish. Our findings reveal that reserved progenitors are a significant and complementary source of osteoblasts for zebrafish bone regeneration.

引用

页码：643 / +

页数：11

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