HPLC determination and pharmacokinetic study of valdecoxib in human plasma

被引:16
作者
Sane, RT [1 ]
Menon, S
Deshpande, AY
Jain, A
机构
[1] Ramnarain Ruia Coll, Dept Chem, Bombay 400019, Maharashtra, India
[2] Therapeut Drug Monitoring Lab, Bombay 400022, Maharashtra, India
关键词
column liquid chromatography; valdecoxib; rofecoxib; human plasma;
D O I
10.1365/s10337-004-0442-2
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A sensitive, simple, and accurate high-performance liquid chromatographic method has been developed for determination of valdecoxib and the internal standard rofecoxib in human plasma. Protein was precipitated from plasma samples by addition of perchloric acid (HClO4); the drug was then extracted with diethyl ether. Separation was performed on a Cosmosil C-18 column (150 mm x 4.6 mm i.d., 5 mum particles) with ammonium acetate buffer-acetonitrile, 60:40 (v/v), containing 0.1 % TEA, pH 6.5, as mobile phase. Detection and quantification were performed by UV-visible detection at 239 nm. Detection and quantification limits were 3 and 5 ng mL(-1), respectively. The linear concentration range for valdecoxib was 5-400 ng mL(-1). The validated RP HPLC method was used for determination of the pharmacokinetic data for the drug in humans.
引用
收藏
页码:137 / 141
页数:5
相关论文
共 14 条
[1]  
*ACS, 2000, HOW DEV VAL TROUBL G
[2]  
[Anonymous], QUALITY ASSURANCE PH
[3]  
Chow S.C, 2000, DESIGN ANAL BIOAVAIL
[4]  
DEASY T, 1976, QAULITY CONTROL MED
[5]  
Kondo T, 1996, J MASS SPECTROM, V31, P873, DOI 10.1002/(SICI)1096-9888(199608)31:8<873::AID-JMS368>3.3.CO
[6]  
2-A
[7]  
SHAH VP, 1991, EUR J DRUG METAB PH, V16, P11
[8]  
SMYTH MR, 1992, SCH CHEM SCI
[9]  
SPRIET A, 1987, EUR J DRUG METAB PH, V27, P109
[10]   Determination of candesartan cilexetil, candesartan and a metabolite in human plasma and urine by liquid chromatography and fluorometric detection [J].
Stenhoff, H ;
Lagerström, PO ;
Andersen, C .
JOURNAL OF CHROMATOGRAPHY B, 1999, 731 (02) :411-417