Immunodiagnosis of human fascioliasis by an enzyme-linked immunosorbent assay (ELISA) and a micro-ELISA

被引:43
作者
Carnevale, S [1 ]
Rodríguez, MI [1 ]
Santillán, G [1 ]
Labbé, JH [1 ]
Cabrera, MG [1 ]
Bellegarde, EJ [1 ]
Velásquez, JN [1 ]
Trgovcic, JE [1 ]
Guarnera, EA [1 ]
机构
[1] ANLIS Dr Carlos G Malbran, Inst Nacl Parasitol, Dept Parasitol Sanitaria, RA-1281 Buenos Aires, DF, Argentina
关键词
D O I
10.1128/CDLI.8.1.174-177.2001
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Enzyme-Linked immunosorbent assay (ELISA) and micro-ELISA were evaluated for their ability to detect anti-Fasciola hepatica antibodies in humans by using excretory-secretory antigen. The sensitivity of each method was 100%, but the specificity was 100% for ELISA and 97% for micro-ELISA. The micro-ELISA could be used as a screening assay and ELISA could be used as a confirmatory method for the serodiagnosis of human fascioliasis.
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收藏
页码:174 / 177
页数:4
相关论文
共 27 条
[1]  
APT W, 1992, REV MED CHILE, V120, P621
[2]   FASCIOLIASIS IN DEVELOPED-COUNTRIES - A REVIEW OF CLASSIC AND ABERRANT FORMS OF THE DISEASE [J].
ARJONA, R ;
RIANCHO, JA ;
AGUADO, JM ;
SALESA, R ;
GONZALEZMACIAS, J .
MEDICINE, 1995, 74 (01) :13-23
[3]   AN OUTBREAK OF ACUTE FASCIOLIASIS AMONG AYMARA INDIANS IN THE BOLIVIAN ALTIPLANO [J].
BJORLAND, J ;
BRYAN, RT ;
STRAUSS, W ;
HILLYER, GV ;
MCAULEY, JB .
CLINICAL INFECTIOUS DISEASES, 1995, 21 (05) :1228-1233
[4]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[5]  
Bryan RT, 1995, GASTROINTESTINAL HEP, P405
[6]  
CAPRON A., 1964, PRESSE MED, V72, P3103
[7]  
Chen M.G., 1990, Tropical diseases bulletin, V87, pR1
[8]   ISOLATION OF FASCIOLA-HEPATICA GENUS-SPECIFIC ANTIGENS [J].
DEWEIL, NS ;
HILLYER, GV ;
PACHECO, E .
INTERNATIONAL JOURNAL FOR PARASITOLOGY, 1984, 14 (02) :197-206
[9]   Dynamics of antigenemia and coproantigens during a human Fasciola hepatica outbreak [J].
Espino, AM ;
Díaz, A ;
Pérez, A ;
Finlay, CM .
JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (09) :2723-2726
[10]   IMMUNODIAGNOSIS OF HUMAN FASCIOLIASIS BY ENZYME-LINKED-IMMUNOSORBENT-ASSAY USING EXCRETORY-SECRETORY PRODUCTS [J].
ESPINO, AM ;
DUMENIGO, BE ;
FERNANDEZ, R ;
FINLAY, CM .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1987, 37 (03) :605-608