ENDOR of metalloenzymes

被引：73

作者：

Hoffman, BM ^{[1
]}

机构：

[1] Northwestern Univ, Dept Chem, Evanston, IL 60208 USA

来源：

ACCOUNTS OF CHEMICAL RESEARCH | 2003年 / 36卷 / 07期

关键词：

NUCLEAR DOUBLE-RESONANCE; COLI RIBONUCLEOTIDE REDUCTASE; TYROSYL RADICAL-DIIRON(III) COFACTOR; ECHO ENVELOPE MODULATION; LYASE ACTIVATING ENZYME; Q-BAND ENDOR; S-ADENOSYLMETHIONINE; HEME OXYGENASE; PULSED ENDOR; SPECTROSCOPY;

D O I：

10.1021/ar0202565

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

This Account examines the role of electron-nuclear double resonance (ENDOR) spectroscopy in furthering our understanding of how metal ions function in biological systems. It briefly describes ENDOR and electron spin-echo envelope modulation (ESEEM) spectroscopies and then illustrates the uses Of ENDOR with several case studies from our own research: cytochrome c peroxidase compound ES; ribonucleotide reductase intermediate X; allylbenzene-inactivated chloroperoxidase; the role of the [4Fe-4S](+) cluster in enzymes of the "radical S-adenosylmethionine" superfamily; di-oxygen activation by heme enzymes. Finally, it briefly considers future developments.

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页码：522 / 529

页数：8

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