Development and Validation of an UPLC-DAD-MS Method for the Determination of Leonurine in Chinese Motherwort (Leonurus japonicus)

被引：20

作者：

Chen, Zhe ^{[1
]}

Wu, Jian Bing ^{[2
]}

Liao, Xiao Juan ^{[1
]}

Yang, Wei ^{[1
]}

Song, Kang ^{[1
]}

机构：

[1] Zhejiang Chinese Med Univ, Zhejiang Hosp Tradit Chinese Med, Natl Clin Res Base Tradit Chinese Med, Hangzhou 310006, Zhejiang, Peoples R China

[2] Zhejiang Univ, Inst Biotechnol, Hangzhou 310029, Zhejiang, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHIC SCIENCE | 2010年 / 48卷 / 10期

关键词：

D O I：

10.1093/chromsci/48.10.802

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

In the present study, an ultra-performance liquid chromatography method with diode array detection (UPLC-DAD) was developed for the analysis and determination of leonurine from motherwort (Leonurus japonicus), a traditional Chinese medicinal plant. This method was validated in terms of specificity, linearity (R2 = 0.9995, linear range: 0.005 ∼ 0.5 mg/mL), precision (< 5.0% RSD), and recovery (103.2%). The extracted amount of leonurine is 0.15 mg/g. Moreover, the target analyte was identified or tentatively characterized using UPLC coupled with electrospary tandem mass spectrometry (UPLC-ESI-MS).

引用

页码：802 / 806

页数：5

共 21 条

[1]

[Anonymous], 2005, INT C HARM ICH Q2 R1

[2]

Labdane diterpenes of Leonurus sibiricus [J].