MouSensor: A Versatile Genetic Platform to Create Super Sniffer Mice for Studying Human Odor Coding

被引：21

作者：

D'Hulst, Charlotte ^{[1
,2
,3
,4
]}

Mina, Raena B. ^{[1
,2
,3
,4
]}

Gershon, Zachary ^{[1
,2
,3
,4
]}

Jamet, Sophie ^{[1
,2
,3
,4
]}

Cerullo, Antonio ^{[1
,2
,3
,4
]}

Tomoiaga, Delia ^{[1
,2
,3
,4
]}

Bai, Li ^{[5
]}

Belluscio, Leonardo ^{[5
]}

Rogers, Matthew E. ^{[6
]}

Sirotin, Yevgeniy ^{[7
]}

Feinstein, Paul ^{[1
,2
,3
,4
]}

机构：

[1] CUNY Hunter Coll, Dept Biol Sci, New York, NY 10065 USA

[2] CUNY, Grad Ctr, Biochem Program, New York, NY 10065 USA

[3] CUNY, Grad Ctr, Biol Program, New York, NY 10065 USA

[4] CUNY, Grad Ctr, Biopsychol & Behav Neurosci Program, New York, NY 10065 USA

[5] NINDS, Dev Neural Plast Sect, NIH, Bethesda, MD 20892 USA

[6] Firmenich Inc, Corp Res & Dev, Plainsboro, NJ 08536 USA

[7] Rockefeller Univ, Shelby White & Leon Levy Ctr Neurobiol & Behav, New York, NY 10065 USA

来源：

CELL REPORTS | 2016年 / 16卷 / 04期

关键词：

OLFACTORY SENSORY NEURONS; RECEPTOR EXPRESSION; AXON GUIDANCE; IN-VIVO; BULB; SYSTEM; SENSITIVITY; MODEL; SCALE;

D O I：

10.1016/j.celrep.2016.06.047

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Typically, similar to 0.1% of the total number of olfactory sensory neurons (OSNs) in the main olfactory epithelium express the same odorant receptor (OR) in a singular fashion and their axons coalesce into homotypic glomeruli in the olfactory bulb. Here, we have dramatically increased the total number of OSNs expressing specific cloned OR coding sequences by multimerizing a 21-bp sequence encompassing the predicted homeodomain binding site sequence, TAATGA, known to be essential in OR gene choice. Singular gene choice is maintained in these "MouSensors.'' In vivo synaptopHluorin imaging of odor-induced responses by known M71 ligands shows functional glomerular activation in an M71 MouSensor. Moreover, a behavioral avoidance task demonstrates that specific odor detection thresholds are significantly decreased in multiple transgenic lines, expressing mouse or human ORs. We have developed a versatile platform to study gene choice and axon identity, to create biosensors with great translational potential, and to finally decode human olfaction.

引用

页码：1115 / 1125

页数：11

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