Multilocus genotypic analysis of Cryptosporidium parvum isolates from different hosts and geographical origins

被引:110
作者
Spano, F
Putignani, L
Crisanti, A
Sallicandro, P
Morgan, UM
Le Blancq, SM
Tchack, L
Tzipori, S
Widmer, G
机构
[1] Tufts Univ, Sch Vet Med, Div Infect Dis, North Grafton, MA 01536 USA
[2] Univ Roma La Sapienza, Ist Parassitol, I-00185 Rome, Italy
[3] Ist Super Sanita, Lab Biol Cellulare, I-00161 Rome, Italy
[4] Univ London Imperial Coll Sci Technol & Med, Dept Biol, London SW7 2BB, England
[5] Murdoch Univ, Div Vet & Biomed Sci, WHO, Collaborating Ctr Mol Epidemiol Infect Dis, Murdoch, WA 6150, Australia
[6] Columbia Univ, Ctr Environm Res Conservat, New York, NY 10032 USA
关键词
D O I
10.1128/JCM.36.11.3255-3259.1998
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The genetic analysis of oocysts recovered from the stools of humans and animals infected with Cryptosporidium parvum has consistently shown the existence of two distinct genotypes, One of the genotypes is found exclusively in some human infections, whereas the other genotype is found in human as well as in animal infections. On the basis of these observations and the results of published epidemiological studies with single polymorphic markers, the existence of two separate transmission cycles has been postulated, one exclusively anthroponotic and the other involving both animals and humans. To test this hypothesis, C. parvum isolates of different geographic and host origins were analyzed by using unlinked genetic polymorphisms. A total of 28 isolates originating from Europe, North and South America, and Australia were examined. Isolates clustered into two groups, one comprising both human and animal isolates and the other comprising isolates only of human origin. The absence of recombinant genotypes is consistent with two reproductively isolated populations within the species C. parvum.
引用
收藏
页码:3255 / 3259
页数:5
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