IL-4 and IL-13 activate the JAK2 tyrosine kinase and Stat6 in cultured human vascular endothelial cells through a common pathway that does not involve the gamma(c) chain

被引:89
作者
PalmerCrocker, RL [1 ]
Hughes, CCW [1 ]
Pober, JS [1 ]
机构
[1] YALE UNIV,SCH MED,BOYER CTR MOL MED,MOL CARDIOBIOL PROGRAM,NEW HAVEN,CT 06536
关键词
cytokines; inflammation; Janus kinases; transcription factors; vascular cell adhesion molecule-1;
D O I
10.1172/JCI118829
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
IL-4 and IL-13 each act on human endothelial cells (ECs) to induce expression of vascular cell adhesion molecule-1. On hematopoietic cells, IL-4 responses may be mediated either through a pathway involving g(c), the common signaling subunit of the IL-2, IL-4, IL-7, IL-9, and IL-15 receptors, or through a g(c)-independent pathway that may be alternatively activated by IL-13. We find that human ECs do not express g(c), as detected by indirect immunofluorescence and FACS(R) analysis or by a reverse transcription-PCR method. Like IL-4, IL-13 activates a protein tyrosine kinase that phosphorylates the IL-4R binding protein. In addition, we find that IL-4 and IL-13 each induce tyrosine phosphorylation of the JAK2 tyrosine kinase, Furthermore, both IL-4 and IL-13 induce binding of the Stat6 transcription factor to a consensus sequence oligonucleotide, We conclude that the IL-4 response of human ECs involves the IL-13 shared pathway that is independent of g(c), and uses JAK2-Stat6 signaling.
引用
收藏
页码:604 / 609
页数:6
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