Replication timing of the human genome

被引:259
作者
Woodfine, K
Fiegler, H
Beare, DM
Collins, JE
McCann, OT
Young, BD
Debernardi, S
Mott, R
Dunham, I
Carter, NP
机构
[1] Wellcome Trust Sanger Inst, Cambridge CB10 1SA, England
[2] St Bartholomews Hosp, Canc Res UK, Mol Oncol Grp, Med Oncol Unit, London, England
[3] Wellcome Trust Ctr Human Genet, Oxford, England
基金
英国医学研究理事会; 英国惠康基金;
关键词
D O I
10.1093/hmg/ddh016
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have developed a directly quantitative method utilizing genomic clone DNA microarrays to assess the replication timing of sequences during the S phase of the cell cycle. The genomic resolution of the replication timing measurements is limited only by the genomic clone size and density. We demonstrate the power of this approach by constructing a genome-wide map of replication timing in human lymphoblastoid cells using an array with clones spaced at 1 Mb intervals and a high-resolution replication timing map of 22q with an array utilizing overlapping sequencing tile path clones. We show a positive correlation, both genome-wide and at a high resolution, between replication timing and a range of genome parameters including GC content, gene density and transcriptional activity.
引用
收藏
页码:191 / 202
页数:12
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