Introducing surface-tethered poly(acrylic acid) brushes as 3D functional thin film for biosensing applications

被引:31
作者
Akkahat, Piyaporn [2 ,3 ]
Hoven, Voravee P. [1 ]
机构
[1] Chulalongkorn Univ, Fac Sci, Dept Chem, Organ Synth Res Unit, Bangkok 10330, Thailand
[2] Chulalongkorn Univ, Fac Sci, Program Petrochem & Polymer Sci, Bangkok 10330, Thailand
[3] Chulalongkorn Univ, Ctr Petr Petrochem & Adv Mat, Bangkok 10330, Thailand
关键词
Polymer brushes; Poly(acrylic acid); Surface-initiated polymerization; Atom transfer radical polymerization; Biotin; Streptavidin; TRANSFER RADICAL POLYMERIZATION; PLASMON RESONANCE SPECTROSCOPY; QUARTZ-CRYSTAL MICROBALANCE; SELF-ASSEMBLED MONOLAYERS; INITIATED POLYMERIZATIONS; METHACRYLATE) BRUSHES; SILICON SURFACES; IMMOBILIZATION; PROTEINS; DENSITY;
D O I
10.1016/j.colsurfb.2011.03.042
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Carboxyl groups of surface-tethered poly(acrylic acid) (PM) brushes should be able to serve as versatile moieties for a wide range of chemical modifications, including an attachment of bioactive species that can act as sensing probes for biosensors. In this research, poly(tert-butyl acrylate) (Pt-BA) brushes were prepared by surface-initiated atom transfer radical polymerization of tert-butyl acrylate. PAA brushes were then obtained after removal of the tert-butyl groups from the Pt-BA brushes by acid hydrolysis. The carboxyl group density of the PM brushes can be varied as a function of chain length or molecular weight. The reactivity of the carboxyl groups of PM brushes towards the immobilization of biotin, a frequently used model bioactive probe in biosensing applications, was evaluated. Qualitative determination of streptavidin (SA) binding to the biotin-attached PM brushes was verified by fluorescence microscopy. The efficiency of the PAA brushes to act as a three dimensional (3D) precursor layer for biosensing applications was further demonstrated using surface plasmon resonance (SPR), where the biotin-attached PM brushes showed an enhanced signal for the biospecific binding of SA in comparison with a self-assembled monolayer (SAM) of a carboxyl-terminated alkanethiol, used as a model two-dimensional (2D) conventional precursor layer. The PM brushes showed very low non-specific interactions with two other tested proteins of a similar pl but different sizes. This desirable feature should be highly beneficial for the development of biosensors. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码:198 / 205
页数:8
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