Isolation, characterization and structural determination of a unique type of arabinogalactan from an immunostimulatory extract of Chlorella pyrenoidosa

被引：114

作者：

Suárez, ER

Kralovec, JA

Noseda, MD

Ewart, HS

Barrow, CJ

Lumsden, MD

Grindley, TB ^{[1
]}

机构：

[1] Dalhousie Univ, Dept Chem, Halifax, NS B3H 4J3, Canada

[2] Ocean Nutr Canada Ltd, Dartmouth, NS B2Y 4T6, Canada

[3] Univ Fed Parana, Biochem & Mol Biol Dept, Curitiba, Parana, Brazil

来源：

CARBOHYDRATE RESEARCH | 2005年 / 340卷 / 08期

关键词：

Chlorella pyrenoidosa; immunomodulators; NMR spectroscopy; polysaccharide structure determination; arabinogalactans;

D O I：

10.1016/j.carres.2005.04.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

An arabinogalactan was isolated from a hot water extract of freeze-dried cells of the green microalga, Chlorella pyrenoidosa. This hot water extract is a proprietary immunomodulator, with the trademark Respondin (TM) (ONC-107). The arabinogalactan was recovered from the ethanol-soluble fraction of the supernatant resulting from a process that involved controlled ethanol precipitation followed by size exclusion chromatography on Sephadex G-100, then Cetavlon precipitation. Sugar analyses, GC-MS data for (S)-2-octyl glycosides, and 1D and 2D NMR experiments established unambiguously that the repeating unit was -> 2)-alpha-L-Araf-(1 -> 3)-[alpha-L-Araf-(1 -> 4)]-beta-D-Galp-(1 ->. This structure does not fit into any of the known classes of arabinogalactans. SEC/MALS experiments gave a molecular mass for the arabinogalactan isolated as 47 +/- 4 kDa but the original structure was probably larger. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：1489 / 1498

页数：10

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