C-erbB3 and c-erbB4 expression is a feature of the endocrine responsive phenotype in clinical breast cancer

被引:105
作者
Knowlden, JM
Gee, JMW
Seery, LT
Farrow, L
Gullick, WJ
Ellis, IO
Blamey, RW
Robertson, JFR
Nicholson, RI
机构
[1] Univ Wales, Coll Med, Tenovus Canc Res Ctr, Cardiff CF4 4XX, S Glam, Wales
[2] Hammersmith Hosp, Imperial Canc Res Fund, Mol Oncol Unit, London W12 0NN, England
[3] City Hosp, Dept Histopathol, Nottingham NG5 1PB, England
[4] City Hosp, Dept Surg, Nottingham NG5 1PB, England
关键词
c-erbB3; c-erbB4; ER; EGFR; cell proliferation; endocrine response; RT-PCR;
D O I
10.1038/sj.onc.1202107
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We examined c-erbB3 and c-erbB4 mRNA expression in 47 primary breast cancer samples by simultaneous RT-PCR and have investigated correlations between these parameters and the expression of both ER and EGFR mRNA and protein as measured by RT-PCR and ICA and with Ki67 immunostaining, A direct association was found between c-erbB3 and c-erbB4 mRNA and ER marker status measured by either RT-PCR (c-erbB3 P = 0.0003; c-erbB4 P = 0.02) or ICA (c-erbB-3 P = 0.002; c-erbB4 P = 0.01). Inverse associations were seen between c-erbB3 and c-erbB4 mRNA marker status and EGFR membrane protein (c-erbB3: P = 0.003; c-erbB4: P = 0.003) and mRNA (c-erbB4: P = 0.009) status. These associations were reinforced by Spearman Rank Correlation Tests. A significant relationship was seen between Ki67 and c-erbB4 mRNA status and level. Measurements of c-erbB3 protein levels in tumour samples removed from a further 89 patients of known response to endocrine therapy: (i) confirmed the relationship between c-erbB3 and ER and (ii) identified that patients whose ER positive tumours expressed high levels of c-erbB3 were most likely to benefit from endocrine measures. A non-significant trend was recorded between c-erbB3 levels and Ki67 immunostaining, These results clearly demonstrate that increased c-erbB3 and c-erbB4 expression appears to be associated with the prognostically-favourable ER phenotype.
引用
收藏
页码:1949 / 1957
页数:9
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