Expression of PTEN in renal cell carcinoma and its relation to tumor behavior and growth

被引:74
作者
Lee, JS
Kim, HS
Kim, YB
Lee, MC
Park, CS
机构
[1] Seonam Univ, Coll Med, Dept Pathol, Namwon 590711, South Korea
[2] Seonam Univ, Coll Med, Dept Surg, Namwon, South Korea
[3] Chonnam Natl Univ, Sch Med, Dept Pathol, Gwangju, South Korea
[4] Res Inst Med Sci, Gwangju, South Korea
关键词
renal cell carcinoma; PTEN; cell proliferation; apoptosis;
D O I
10.1002/jso.10302
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Background and Objectives: PTEN is a candidate tumor suppressor gene in a variety of malignant tumors, including renal cell carcinoma (RCC). PTEN regulates cell cycle progression and cell survival in vivo. However, the role of PTEN alterations and their association with tumor growth and behavior in patients with RCC has not been well established. The aim of our study was to evaluate PTEN expression in RCC and its correlation with clinicopathologic features, cell proliferation, and apoptosis. Methods: Sixty-seven RCC specimens were examined immunohistochemically with anti-PTEN antibody. The apoptotic cells were visualized by terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick-end labeling (TUNEL) and proliferative cells were visualized by staining with Ki-67 antibody. Results: Twenty-one (31.3%) of the 67 RCCs showed reduced PTEN expression. The apoptotic index (AI) varied from 0.2 to 25.5%, and the Ki-67 index (KI) ranged from 1.6 to 69.8%. Reduced PTEN expression correlated with TNM stage (P < 0.05) and nuclear grade (P < 0.05). Tumors with reduced PTEN expression had a significantly higher KI than those with normal PTEN expression (P < 0.01). By univariate analysis, nuclear grade (P = 0.0005), TNM stage (P < 0.0001), AI (P = 0.0220), KI (P = 0.0002), and reduced PTEN expression (P < 0.0001) were associated with shortened survival. However, TNM stage was the only independent prognostic factors by multivariate analysis (P = 0.0007). Conclusions: Our results suggest that PTEN expression is frequently reduced in advanced RCC. The PTEN gene seems to be important for the growth suppression of RCC, by inhibiting cell proliferation. (C) 2003 Wiley-Liss, Inc.
引用
收藏
页码:166 / 172
页数:7
相关论文
共 35 条
[1]  
Alimov A, 1999, ANTICANCER RES, V19, P3841
[2]  
*ASS DIR AN SURG P, 1996, HUM PATHOL, V27, P1005
[3]   Reduced expression of PTEN correlates with breast cancer progression [J].
Bose, S ;
Crane, A ;
Hibshoosh, H ;
Mansukhani, M ;
Sandweis, L ;
Parsons, R .
HUMAN PATHOLOGY, 2002, 33 (04) :405-409
[4]   Loss of tumor suppressor protein PTEN during renal carcinogenesis [J].
Brenner, W ;
Färber, G ;
Herget, T ;
Lehr, HA ;
Hengstler, JG ;
Thüroff, JW .
INTERNATIONAL JOURNAL OF CANCER, 2002, 99 (01) :53-57
[5]   Point mutation and homozygous deletion of PTEN/MMAC1 in primary bladder cancers [J].
Cairns, P ;
Evron, E ;
Okami, K ;
Halachmi, N ;
Esteller, M ;
Herman, JG ;
Bose, S ;
Wang, SI ;
Parsons, R ;
Sidransky, D .
ONCOGENE, 1998, 16 (24) :3215-3218
[6]   PROGNOSTIC-SIGNIFICANCE OF MORPHOLOGIC PARAMETERS IN RENAL-CELL CARCINOMA [J].
FUHRMAN, SA ;
LASKY, LC ;
LIMAS, C .
AMERICAN JOURNAL OF SURGICAL PATHOLOGY, 1982, 6 (07) :655-663
[7]  
Furnari FB, 1998, CANCER RES, V58, P5002
[8]   IDENTIFICATION OF PROGRAMMED CELL-DEATH INSITU VIA SPECIFIC LABELING OF NUCLEAR-DNA FRAGMENTATION [J].
GAVRIELI, Y ;
SHERMAN, Y ;
BENSASSON, SA .
JOURNAL OF CELL BIOLOGY, 1992, 119 (03) :493-501
[9]  
Guinan P, 1997, CANCER, V80, P992, DOI 10.1002/(SICI)1097-0142(19970901)80:5<992::AID-CNCR26>3.0.CO
[10]  
2-Q