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Immunoassay using the probe-labeled Au/Ag core-shell nanoparticles based on surface-enhanced Raman scattering

被引：44
作者：
Ji, XH
Xu, SP
Wang, LY
Liu, M
Pan, K
Yuan, H
Ma, L
Xu, WQ
Li, JH [1 ]
Bai, YB
Li, TJ
机构：
[1] Tsinghua Univ, Dept Chem, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Beijing 100084, Peoples R China
[2] Jilin Univ, Coll Chem, Changchun 130023, Peoples R China
[3] Yunnan Univ, Kunming Yunda Monoclonal Antibody Technol Ctr, Kunming 650091, Peoples R China
来源：
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS | 2005年 / 257-58卷
关键词：
Au/Ag core-shell nanoparticles; immunoassay; antibody; antigen; SERS;
D O I：
10.1016/j.colsurfa.2004.10.096
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 [物理化学]; 081704 [应用化学];
摘要：
Au/Ag core-shell nanoparticles labeled by monoclonal antibody and surface-enhanced Raman scattering (SERS) probes were used for immunoassay analysis. Mouse polyclonal antibodies against hepatitis B surface antigen (mouse Anti-HBsAg PAb) were covalently immobilized on the silicon chips modified by a self-assembled monolayer of (3-amino-propyl) trimethoxysilane via glutaraldehyde (GA) activation. The Au/Ag core-shell nanoparticles were labeled by the mouse monoclonal antibody against hepatitis B surface antigen (mouse Anti-HBsAg MAb) and the probe molecule, p-mercaptobenzoic acid (MBA). Immunoreactions between the labeled Au/Ag core-shell nanoparticles and the corresponding antigens captured by the Anti-HBsAg PAb on the silicon chips were investigated by SERS. (c) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码：171 / 175
页数：5
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