Internal motions in myosin head: Effect of ADP and ATP

被引：14

作者：

Belagyi, J ^{[1
]}

Lorinczy, D ^{[1
]}

机构：

[1] UNIV PECS,SCH MED,DEPT BIOPHYS,PECS,HUNGARY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1996年 / 219卷 / 03期

基金：

新加坡国家研究基金会;

关键词：

D O I：

10.1006/bbrc.1996.0336

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Internal flexibility of myosin heads in glycerinated muscle fibres in the presence of MgADP plus orthovanadate and after addition of Ca-ATP was studied using an isothiocyanate-based spin label attached to the reactive sulfhydryl sites of myosin. The spin labels were immobilized on the microsecond time scale and exhibited significant orientational order in rigor. In AM(+).ADP.V-i state a smaller fraction of ordered population was found showing distinct orientation from rigor; the larger population of heads was in dinamically disordered state. This new ordered population of heads was detected even in contracting fibres. (C) 1996 Academic Press, Inc.

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收藏

页码：936 / 940

页数：5

相关论文

共 22 条

[1] PATH AND EXTENT OF CROSS-BRIDGE ROTATION DURING MUSCLE-CONTRACTION [J].

AJTAI, K ;

TOFT, DJ ;

BURGHARDT, TP .

BIOCHEMISTRY, 1994, 33 (18) :5382-5391

[2] SATURATION TRANSFER ELECTRON-PARAMAGNETIC RESONANCE OF SPIN-LABELED MUSCLE-FIBERS - DEPENDENCE OF MYOSIN HEAD ROTATIONAL MOTION ON SARCOMERE-LENGTH [J].

BARNETT, VA ;

THOMAS, DD .

JOURNAL OF MOLECULAR BIOLOGY, 1984, 179 (01) :83-102

[3] MICROSECOND ROTATIONAL MOTION OF SPIN-LABELED MYOSIN HEADS DURING ISOMETRIC MUSCLE-CONTRACTION - SATURATION TRANSFER ELECTRON-PARAMAGNETIC RESONANCE [J].

BARNETT, VA ;

THOMAS, DD .

BIOPHYSICAL JOURNAL, 1989, 56 (03) :517-523

[4] ADP-INDUCED CHANGES IN ORDERING OF SPIN-LABELED MYOSIN HEADS IN MUSCLE-FIBERS [J].

BELAGYI, J ;

FREY, I ;

POTO, L .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1994, 224 (01) :215-222

[5]

BELAGYI J, 1979, STUD BIOPHYS, V77, P77

[6]

Brenner B., 1990, Molecular mechanisms in muscular contraction, P77

[7]

BURGHARDT TP, 1990, MOL MECHANISMS MUSCU, P211

[8] THE EFFECT OF MYOSIN SULFHYDRYL MODIFICATION ON THE MECHANICS OF FIBER CONTRACTION [J].

CROWDER, MS ;

COOKE, R .

JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY, 1984, 5 (02) :131-146

[9] MICROWAVE AND MODULATION FIELD INHOMOGENEITIES AND THE EFFECT OF CAVITY-Q IN SATURATION TRANSFER ELECTRON-SPIN-RESONANCE SPECTRA - DEPENDENCE ON SAMPLE-SIZE [J].

FAJER, P ;

MARSH, D .

JOURNAL OF MAGNETIC RESONANCE, 1982, 49 (02) :212-224

[10] EFFECTS OF AMPPNP ON THE ORIENTATION AND ROTATIONAL-DYNAMICS OF SPIN-LABELED MUSCLE CROSS-BRIDGES [J].

FAJER, PG ;

FAJER, EA ;

BRUNSVOLD, NJ ;

THOMAS, DD .

BIOPHYSICAL JOURNAL, 1988, 53 (04) :513-524