Close relationship between G8-serotype bovine and human rotaviruses isolated in Nigeria

被引：32

作者：

Adah, MI

Nagashima, S

Wakuda, M

Taniguchi, K ^{[1
]}

机构：

[1] Fujita Hlth Univ, Sch Med, Dept Virol & Parasitol, Toyoake, Aichi 4701192, Japan

[2] Univ Maiduguri, Dept Vet Med, Maiduguri, Nigeria

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2003年 / 41卷 / 08期

关键词：

D O I：

10.1128/JCM.41.8.3945-3950.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A bovine rotavirus, NGRBg8, isolated from the feces of a calf with diarrhea in Nigeria was characterized by reverse transcription-PCR, nucleotide sequence analysis, and Northern blot hybridization. The nucleotide sequence of the VP7 gene of the strain was most closely related to that of a Nigerian human G8-serotype strain, HMG035 (99.9%). The NSP1 gene of strain NGRBg8 is highly related (99.4%) to that of a Thai G8 bovine strain, A5-10. Northern blot hybridization revealed a high overall genomic relatedness of bovine strain NGRBg8 with human strain HMG035; all 11 RNA segments hybridized to each other. Thus, the results show the close relationship between G8 bovine and human rotaviruses in Nigeria.

引用

页码：3945 / 3950

页数：6

共 44 条

[1] Further characterization of field strains of rotavirus from Nigeria VP4 genotype P6 most frequently identified among symptomatically infected children
Adah, MI
Rohwedder, A
Olaleye, OD
Durojaiye, OA
Werchau, H
[J]. JOURNAL OF TROPICAL PEDIATRICS, 1997, 43 (05) : 267 - 274
[2] Nigerian rotavirus serotype GS could not be typed by PCR due to nucleotide mutation at the 3' end of the primer binding site
Adah, MI
Rohwedder, A
Olaleyle, OD
Werchau, H
[J]. ARCHIVES OF VIROLOGY, 1997, 142 (09) : 1881 - 1887
[3] Molecular epidemiology of rotaviruses in Nigeria: Detection of unusual strains with G2P[6] and G8P[1] specificities
Adah, MI
Wade, A
Taniguchi, K
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (11) : 3969 - 3975
[4] Adah MI, 1997, TROP MED INT HEALTH, V2, P363
[5] Armah GE, 2001, J MED VIROL, V63, P67, DOI 10.1002/1096-9071(200101)63:1&lt
[6] 67::AID-JMV1010&gt
[7] 3.0.CO
[8] 2-T
[9] HUMAN AND BOVINE SEROTYPE G8 ROTAVIRUSES MAY BE DERIVED BY REASSORTMENT
BROWNING, GF
SNODGRASS, DR
NAKAGOMI, O
KAGA, E
SARASINI, A
GERNA, G
[J]. ARCHIVES OF VIROLOGY, 1992, 125 (1-4) : 121 - 128
[10] The characterization of VP7 (G type) and VP4 (P type) genes of bovine group A rotaviruses from field samples using RT-PCR and RFLP analysis
Chang, KO
Parwani, AV
Saif, LJ
[J]. ARCHIVES OF VIROLOGY, 1996, 141 (09) : 1727 - 1739

← 1 2 3 4 5 →