Comparison of Staphylococcus aureus isolates from food handlers, raw bovine milk and Minas Frescal cheese by antibiogram and pulsed-field gel electrophoresis following SmaI digestion

被引:85
作者
Andre, Maria Claudia D. P. B. [1 ]
Hidalgo Campos, Maria Raquel [1 ]
Borges, Liana Jayme [1 ]
Kipnis, Andre [1 ]
Pimenta, Fabiana Cristina [1 ]
Serafini, Alvaro Bisol [1 ]
机构
[1] Univ Fed Goias, Inst Patol Trop & Saude Publ, Dept Microbiol Immunol Parasitol & Patol, BR-74605050 Goiania, Go, Brazil
关键词
Staphylococcus aureus; pulsotypes; minas frescal cheese;
D O I
10.1016/j.foodcont.2007.03.010
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
During a year (from February/2004 to March/2005) 140 samples of food handlers (92), raw milk (24) and Minas Frescal cheese (24) were analyzed for the presence of Staphylococcus aureus in a dairy processing plant of Goias State, Brazil. Seventy-three S. aureus isolates were obtained and compared by antimicrobial susceptibility and by the DNA macrorestriction patterns obtained from pulsed-field gel electrophoresis following SmaI digestion in order to investigate the possible sources of cheeses contamination. The results showed high diversity among the strains, demonstrating a lack of predominance of an endemic clone in the dairy plant. Based on the results, the raw milk appears to be the most probable source of S. aureus contamination in cheeses. (c) 2007 Elsevier Ltd. All rights reserved.
引用
收藏
页码:200 / 207
页数:8
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