A rapid screening approach to metabonomics using UPLC and oa-TOF mass spectrometry: application to age, gender and diurnal variation in normal/Zucker obese rats and black, white and nude mice

被引：188

作者：

Plumb, RS

Granger, JH

Stumpf, CL

Johnson, KA

Smith, BW

Gaulitz, S

Wilson, ID

Castro-Perez, J

机构：

[1] Waters Corp, Milford, MA 01757 USA

[2] AstraZeneca, Dept Drug Metab & Pharmacokinet, Macclesfield SK10 4TG, Cheshire, England

[3] Waters Corp, Manchester M23 9LZ, Lancs, England

来源：

ANALYST | 2005年 / 130卷 / 06期

关键词：

D O I：

10.1039/b501767j

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The use of Ultra Performance Liquid Chromatography (UPLC), with a rapid 1.5 minute reversed-phase gradient separation on a 1.7 mu m reversed-phase packing material to provide rapid "high throughput" support for metabonomic screening is demonstrated. The peak capacity and the number of marker ions detected using these fast UPLC separations and oa-TOF MS was found to be similar to that generated by conventional HPLC-MS methods with a 10 minute separation. The UPLC-MS methodology was applied to the analysis of urine samples from rodents, including normal and Zucker obese rats and three strains of mice (of both sexes), and was found to provide rapid discrimination between age, strain, gender and diurnal variation.

引用

页码：844 / 849

页数：6

共 23 条

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