A Conserved Coatomer-related Complex Containing Sec13 and Seh1 Dynamically Associates With the Vacuole in Saccharomyces cerevisiae

被引：106

作者：

Dokudovskaya, Svetlana ^{[1
]}

Waharte, Francois ^{[2
]}

Schlessinger, Avner ^{[3
,4
]}

Pieper, Ursula ^{[3
,4
]}

Devos, Damien P. ^{[5
]}

Cristea, Ileana M. ^{[6
]}

Williams, Rosemary ^{[7
]}

Salamero, Jean ^{[2
]}

Chait, Brian T. ^{[8
]}

Sali, Andrej ^{[3
,4
]}

Field, Mark C. ^{[9
]}

Rout, Michael P. ^{[5
,7
]}

Dargemont, Catherine ^{[1
,5
]}

机构：

[1] CNRS, Inst Jacques Monod, UMR 7592, F-75205 Paris 13, France

[2] Inst Curie, CNRS, Cell & Tissue Imaging PICT IBiSA, UMR 144, F-75248 Paris 05, France

[3] Univ Calif San Francisco, Dept Bioengn & Therapeut Sci, Dept Pharmaceut Chem, San Francisco, CA 94158 USA

[4] Univ Calif San Francisco, Calif Inst Quantitat Biosci QB3, San Francisco, CA 94158 USA

[5] EMBL, D-69117 Heidelberg, Germany

[6] Princeton Univ, Dept Mol Biol, Lewis Thomas Lab, Princeton, NJ 08544 USA

[7] Rockefeller Univ, Lab Cellular & Struct Biol, New York, NY 10065 USA

[8] Rockefeller Univ, Lab Mass Spectrometry & Gaseous Ion Chem, New York, NY 10065 USA

[9] Univ Cambridge, Dept Pathol, Cambridge CB2 1QT, England

来源：

MOLECULAR & CELLULAR PROTEOMICS | 2011年 / 10卷 / 06期

关键词：

NUCLEAR-PORE COMPLEX; PROTEIN-STRUCTURE PREDICTION; TUMOR-SUPPRESSOR NPRL2; COATED VESICLES; COPII; EVOLUTION; DOMAIN; ARCHITECTURE; YEAST; IDENTIFICATION;

D O I：

10.1074/mcp.M110.006478

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The presence of multiple membrane-bound intracellular compartments is a major feature of eukaryotic cells. Many of the proteins required for formation and maintenance of these compartments share an evolutionary history. Here, we identify the SEA (Seh1-associated) protein complex in yeast that contains the nucleoporin Seh1 and Sec13, the latter subunit of both the nuclear pore complex and the COPII coating complex. The SEA complex also contains Npr2 and Npr3 proteins (upstream regulators of TORC1 kinase) and four previously uncharacterized proteins (Sea1-Sea4). Combined computational and biochemical approaches indicate that the SEA complex proteins possess structural characteristics similar to the membrane coating complexes COPI, COPII, the nuclear pore complex, and, in particular, the related Vps class C vesicle tethering complexes HOPS and CORVET. The SEA complex dynamically associates with the vacuole in vivo. Genetic assays indicate a role for the SEA complex in intracellular trafficking, amino acid biogenesis, and response to nitrogen starvation. These data demonstrate that the SEA complex is an additional member of a family of membrane coating and vesicle tethering assemblies, extending the repertoire of protocoatomer-related complexes. Molecular & Cellular Proteomics 10: 10.1074/mcp.M110.006478, 1-17, 2011.

引用

页数：17

共 75 条

[1] The molecular architecture of the nuclear pore complex [J].

Alber, Frank ;

Dokudovskaya, Svetlana ;

Veenhoff, Liesbeth M. ;

Zhang, Wenzhu ;

Kipper, Julia ;

Devos, Damien ;

Suprapto, Adisetyantari ;

Karni-Schmidt, Orit ;

Williams, Rosemary ;

Chait, Brian T. ;

Sali, Andrej ;

Rout, Michael P. .

NATURE, 2007, 450 (7170) :695-701

[2] Determining the architectures of macromolecular assemblies [J].

Alber, Frank ;

Dokudovskaya, Svetlana ;

Veenhoff, Liesbeth M. ;

Zhang, Wenzhu ;

Kipper, Julia ;

Devos, Damien ;

Suprapto, Adisetyantari ;

Karni-Schmidt, Orit ;

Williams, Rosemary ;

Chait, Brian T. ;

Rout, Michael P. ;

Sali, Andrej .

NATURE, 2007, 450 (7170) :683-694

[3] Total Internal Reflection Fluorescence Microscopy [J].

Axelrod, Daniel .

BIOPHYSICAL TOOLS FOR BIOLOGISTS, VOL 2: IN VIVO TECHNIQUES, 2008, 89 :169-221

[4] Crystal structure of the Sec18p N-terminal domain [J].

Babor, SM ;

Fass, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (26) :14759-14764

[5]

Ballon DR, 2006, CELL, V126, P1079, DOI 10.1016/j.cell.2006.07.030

[6]

Bateman A, 2004, NUCLEIC ACIDS RES, V32, pD138, DOI [10.1093/nar/gkp985, 10.1093/nar/gkh121, 10.1093/nar/gkr1065]

[7] Structure of the Sec23/24-Sar1 pre-budding complex of the COPII vesicle coat [J].

Bi, XP ;

Corpina, RA ;

Goldberg, J .

NATURE, 2002, 419 (6904) :271-277

[8] The mechanisms of vesicle budding and fusion [J].

Bonifacino, JS ;

Glick, BS .

CELL, 2004, 116 (02) :153-166

[9] Patch-Based Nonlocal Functional for Denoising Fluorescence Microscopy Image Sequences [J].

Boulanger, Jerome ;

Kervrann, Charles ;

Bouthemy, Patrick ;

Elbau, Peter ;

Sibarita, Jean-Baptiste ;

Salamero, Jean .

IEEE TRANSACTIONS ON MEDICAL IMAGING, 2010, 29 (02) :442-454

[10] A Global Protein Kinase and Phosphatase Interaction Network in Yeast [J].

Breitkreutz, Ashton ;

Choi, Hyungwon ;

Sharom, Jeffrey R. ;

Boucher, Lorrie ;

Neduva, Victor ;

Larsen, Brett ;

Lin, Zhen-Yuan ;

Breitkreutz, Bobby-Joe ;

Stark, Chris ;

Liu, Guomin ;

Ahn, Jessica ;

Dewar-Darch, Danielle ;

Reguly, Teresa ;

Tang, Xiaojing ;

Almeida, Ricardo ;

Qin, Zhaohui Steve ;

Pawson, Tony ;

Gingras, Anne-Claude ;

Nesvizhskii, Alexey I. ;

Tyers, Mike .

SCIENCE, 2010, 328 (5981) :1043-1046

← 1 2 3 4 5 6 7 8 →