Oxymatrine Ameliorates L-Arginine-Induced Acute Pancreatitis in Rats

被引:34
作者
Zhang, Zhiqiang [1 ]
Wang, Yanqing [1 ]
Dong, Ming [2 ]
Cui, Jianchun [1 ]
Rong, Daqing [1 ]
Dong, Qi [1 ]
机构
[1] Peoples Hosp Liaoning Prov, Dept Gen Surg, Shenyang 110016, Peoples R China
[2] China Med Univ, Dept Gen Surg, Affiliated Hosp 1, Shenyang 110001, Peoples R China
基金
中国国家自然科学基金;
关键词
oxymatrine; acute pancreatitis; tight junction proteins; tumor necrosis factor alpha; CD45; INTESTINAL-MUCOSA; ORGAN DYSFUNCTION; DIAMINE OXIDASE; ACINAR-CELLS; HEPATITIS-B; EXPRESSION; INJURY; MECHANISM; MICE; PERMEABILITY;
D O I
10.1007/s10753-011-9352-2
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The aim of this study was to determine whether oxymatrine has a protective effect against acute pancreatitis (AP) in a rat model of L-arginine-induced AP. AP was induced by two intraperitoneal injections of L-arginine (250 mg/100 g) at a 1-h interval. Oxymatrine (50 mg/kg) was administered every 6 h after the induction of AP. Oxymatrine significantly reduced the plasma amylase, D-lactic acid and tumor necrosis factor alpha concentration, serum diamine oxidase and lipase activity, and pancreatic myeloperoxidase activity, which were increased in AP rats (P<0.05). In addition, the pancreatic CD45 expression and the expression of claudin-1, but not zonula occludens-1 (ZO-1) and occludin, in the intestinal tissues were significantly reduced after the induction of AP. However, oxymatrine increased the expression of claudin-1 and CD45, but did not alter the expression of ZO-1 and occludin. In conclusion, our results demonstrated that oxymatrine is potentially capably of protecting against L-arginine-induced AP and attenuating AP-associated intestinal barrier injury by up-regulation of claudin-1.
引用
收藏
页码:605 / 613
页数:9
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