Development of ELISAs for the measurement of the dietary phytoestrogens daidzein and equol in human plasma

被引：13

作者：

Creeke, PI ^{[1
]}

Wilkinson, AP ^{[1
]}

Lee, HA ^{[1
]}

Morgan, MRA ^{[1
]}

Price, KR ^{[1
]}

Rhodes, MJC ^{[1
]}

机构：

[1] Inst Food Res, Dept Biochem, Norwich NR4 7UA, Norfolk, England

来源：

FOOD AND AGRICULTURAL IMMUNOLOGY | 1998年 / 10卷 / 04期

关键词：

daidzein; equol; ELISA; human plasma; phytoestrogens;

D O I：

10.1080/09540109809354996

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Microtitration plate ELISAs were developed for the isoflavane daidzein, a phytoestrogen found mainly in soya and the mammalian metabolite of daidzein, equol. The detection limits in assay buffer were 21 pg per well for daidzein and 70 pg per well for equol. The assays were both highly specific. Human plasma was extracted with ail organic solvent and standard corves for daidzein and equol were obtained in the presence of the extract. Applications of the assays are discussed.

引用

页码：325 / 337

页数：13

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