Exosome-loaded dendritic cells elicit tumor-specific CD8+ cytotoxic T cells in patients with glioma

被引：117

作者：

Bu, Ning ^{[1
]}

Wu, Haiqin ^{[1
]}

Sun, Bingzhong ^{[2
]}

Zhang, Guilian ^{[1
]}

Zhan, Shuqin ^{[1
]}

Zhang, Ru ^{[1
]}

Zhou, Le ^{[3
]}

机构：

[1] Xi An Jiao Tong Univ, Dept Neurol, Affiliated Hosp 2, Xian 710049, Shaanxi, Peoples R China

[2] Fourth Mil Med Univ, Dept Hematol, Xijing Hosp, Xian 710032, Shaanxi, Peoples R China

[3] Xi An Jiao Tong Univ, Dept Neurosurg, Affiliated Hosp 2, Xian 710049, Shaanxi, Peoples R China

来源：

JOURNAL OF NEURO-ONCOLOGY | 2011年 / 104卷 / 03期

关键词：

Exosome; Glioma; Dendritic cells; MALIGNANT GLIOMA; ANTIGEN; VACCINATION; IMMUNOTHERAPY; LYMPHOCYTES; RESPONSES; BLOOD; TRIAL;

D O I：

10.1007/s11060-011-0537-1

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

In this study, we demonstrate that tumor-derived exosome-loaded dendritic cells can elicit a specific CD8(+) cytotoxic T-lymphocyte (CTL) response against autologous tumor cells in patients with malignant glioma. Exosomes were purified by ultrafiltration centrifugation and sucrose gradient ultracentrifugation. Exosomes had antigen-presenting molecules (MHC-I, HSP70), tumor antigen (MAGE-1) and adherent molecule (ICAM-1). After incubation with exosomes, the dendritic cells (DCs) could activate the T lymphocytes to become glioma-specialized CTL. The CTL had vigorous cytotoxicity to glioma cells as opposed to autologous lymphoblast cells. These data demonstrate that tumor exosome-loaded DC can be an effective tool in inducing glioma-specific CD8(+) CTLs able to kill autologous glioma cells in vitro. In conclusion, exosomes are a natural and new source of tumor-rejection antigens, opening up new avenues for immunization against glioma.

引用

页码：659 / 667

页数：9

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