Transcription factor AP-2 activity is modulated by protein kinase A-mediated phosphorylation

被引：61

作者：

García, MA ^{[1
]}

Campillos, M ^{[1
]}

Marina, A ^{[1
]}

Valdivieso, F ^{[1
]}

Vázquez, J ^{[1
]}

机构：

[1] Univ Autonoma Madrid, CSIC, Ctr Biol Mol Severo Ochoa, E-28049 Madrid, Spain

来源：

FEBS LETTERS | 1999年 / 444卷 / 01期

关键词：

AP-2; cAMP; protein kinase A; APOE promoter; Alzheimer's disease;

D O I：

10.1016/S0014-5793(99)00021-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We recently reported that APOE promoter activity is stimulated by cAMP, this effect being mediated by factor AP-2 [Garcia et al. (1996) J. Neurosci. 16, 7550-7556]. Here, we study whether cAMP-induced phosphorylation modulates the activity of AP-2. Recombinant AP-2 was phosphorylated in vitro by protein kinase A (PKA) at Ser(239). Mutation of Ser(239) to Ala abolished in vitro phosphorylation of AP-2 by PKA, but not the DNA binding activity of AP-2. Cotransfection studies showed that PKA stimulated the effect of AP-2 on the APOE promoter, but not that of the S239A mutant, Therefore, cAMP may modulate AP-2 activity by PKA-induced phosphorylation of this factor. (C) 1999 Federation of European Biochemical Societies.

引用

页码：27 / 31

页数：5

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