A technical trick for studying proteomics in parallel to transcriptomics in symbiotic root-fungus interactions

被引:19
作者
Dumas-Gaudot, E
Amiour, N
Weidmann, S
Bestel-Correl, G
Valot, B
Lenogue, S
Gianinazzi-Pearson, V
Gianinazzi, S
机构
[1] CMSE, INRA, Bourgogne Plante Microbe Environm, CNRS,5184U,UMR 1088, F-21065 Dijon, France
[2] ETabol EXplorer, St Beauzire, France
关键词
arbuscular mycorrhiza; functional genomics; Glomus mosseae; Medicago truncatula; proteome analyses;
D O I
10.1002/pmic.200300627
中图分类号
Q5 [生物化学];
学科分类号
071010 [生物化学与分子生物学]; 081704 [应用化学];
摘要
We have developed a protocol in which proteins and mRNA can be analyzed from single root samples. This experimental design was validated in arbuscular mycorrhiza by comparing the proteins profiles obtained with those from a classical protein extraction process. It is a step forward to make simultaneous proteome and transcriptiome profiling possible.
引用
收藏
页码:451 / 453
页数:3
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