Use of a single-mode fiber coupler for second-harmonic-generation microscopy

被引：20

作者：

Fu, L ^{[1
]}

Gan, XS ^{[1
]}

Gu, M ^{[1
]}

机构：

[1] Swinburne Univ Technol, Sch Biophys Sci & Elect Engn, Ctr Microphoton, Hawthorn, Vic 3122, Australia

来源：

OPTICS LETTERS | 2005年 / 30卷 / 04期

关键词：

D O I：

10.1364/OL.30.000385

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We present a compact second-harmonic-generation (SHG) microscope based on a three-port single-mode fiber coupler. The fiber coupler is used to deliver a near-infrared ultrashort-pulsed laser beam as well as to collect the SHG signal in the visible wavelength range. The SHG microscope exhibits an axial resolution of 1.8 mum, which shows a slight enhancement of the optical sectioning effect compared with that for two-photon excitation at the same excitation wavelength. It is also demonstrated that SHG and two-photon fluorescence images under parallel and perpendicular laser excitation polarization can be simultaneously obtained. (C) 2005 Optical Society of America.

引用

页码：385 / 387

页数：3

共 14 条

[1]

Agrawal G., 2019, Nonlinear fiber optics, V6th

[2] Two-photon fluorescence endoscopy with a micro-optic scanning head [J].