Characterization of a newly established human chondrosarcoma cell line, CS-OKB

被引：20

作者：

Chano, T ^{[1
]}

Okabe, H

Saeki, Y

Ishizawa, M

Matsumoto, K

Hukuda, S

机构：

[1] Shiga Univ Med Sci, Dept Orthopaed Surg, Ohtsu, Shiga 5202192, Japan

[2] Shiga Univ Med Sci, Dept Clin Lab Med, Shiga, Japan

[3] Shiga Univ Med Sci, Dept Basic Sci Hlth & Nursing, Ohtsu, Shiga 5202192, Japan

来源：

VIRCHOWS ARCHIV-AN INTERNATIONAL JOURNAL OF PATHOLOGY | 1998年 / 432卷 / 06期

关键词：

human chondrosarcoma; cell line; type II collagen; RT-PCR; immunocytochemistry;

D O I：

10.1007/s004280050201

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

A clonal cell line, CS-OKB, was derived from a human chondrosarcoma and characterized by cytogenetic study, immunocytochemical staining, and reverse transcriptase polymerase chain reaction (RT-PCR). Chromosomal abnormalities characteristic of malignant cartilaginous neoplasms were identified. CS-OKB cells were intensely stained with anti-type II collagen and anti-keratan sulphate antibodies. RT-PCR indicated that CS-OKB transcribes cartilage-specific genes such as type II, X procollagen, and aggrecan. This human chondrosarcoma cell line is stable and expresses well-differentiated chondrocyte-specific genes. It synthesizes well-differentiated chondrocyte-specific molecules in uncoated plastic dishes. CS-OKB may be useful for studies of human chondrocytes and in characterizing human chondrosarcomas.

引用

页码：529 / 534

页数：6

共 20 条

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