Mutations in Drosophila sec15 reveal a function in neuronal targeting for a subset of exocyst components

被引:112
作者
Mehta, SQ
Hiesinger, PR
Beronja, S
Zhal, RG
Schulze, KL
Verstreken, P
Cao, Y
Zhou, Y
Tepass, U
Crair, MC
Bellen, HJ
机构
[1] Baylor Coll Med, Program Dev Biol, Houston, TX 77030 USA
[2] Baylor Coll Med, Howard Hughes Med Inst, Houston, TX 77030 USA
[3] Baylor Coll Med, Dept Mol & Human Genet, Houston, TX 77030 USA
[4] Baylor Coll Med, Dept Neurosci, Houston, TX 77030 USA
[5] Univ Toronto, Dept Zool, Toronto, ON M5S 3G5, Canada
关键词
D O I
10.1016/j.neuron.2005.02.029
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The exocyst is a complex of proteins originally identified in yeast that has been implicated in polarized secretion. Components of the exocyst have been implicated in neurite outgrowth, cell polarity, and cell viability. We have isolated an exocyst component, sec15, in a screen for genes required for synaptic specificity. Loss of sec15 causes a targeting defect of photoreceptors that coincides with mislocalization of specific cell adhesion and signaling molecules. Additionally, sec15 mutant neurons fail to localize other exocyst members like Sec5 and Sec8, but not Sec6, to neuronal terminals. However, loss of sec15 does not cause cell lethality in contrast to loss of sec5 or sec6. Our data suggest a role of Sec15 in an exocyst-like subcomplex for the targeting and subcellular distribution of specific proteins. The data also show that functions of other exocyst components persist in the absence of sec15, suggesting that different exocyst components have separable functions.
引用
收藏
页码:219 / 232
页数:14
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