Synthesis of light-activated antisense oligodeoxynucleotide

被引：23

作者：

Tang, XinJing ^{[1
]}

Dmochowski, Ivan J. ^{[1
]}

机构：

[1] Univ Penn, Dept Chem, Philadelphia, PA 19104 USA

来源：

NATURE PROTOCOLS | 2006年 / 1卷 / 06期

关键词：

D O I：

10.1038/nprot.2006.462

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The activity of a 20-mer antisense oligodeoxynucleotide (asODN) is transiently blocked by attaching a partially complementary sense strand (sODN) via a heterobifunctional photocleavable linker (PL). The asODN-PL-sODN conjugate forms a DNA hairpin-like structure that is considerably more stable than the corresponding asODN/sODN duplex. In conjugate form, the asODN is prevented from hybridizing to exogenous RNA or DNA molecules. Activity is restored after modest exposure to UV light (lambda approximate to 365 nm). Here, we provide a detailed procedure for synthesizing photoactive asODNs in good yields. Synthesis, purification and analysis of the light-activated asODN can be completed within 1-2 weeks.

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收藏

页码：3041 / 3048

页数：8

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